Hello Laura, We use Dako's Mouse Monoclonal Anti-Human HLA-ABC antigen Cat.# M0736 for MHC Class 1
I just looked online and the spec sheet is not available, but here is what the spec sheet says that I have in front of me: Specificity: "Anti-HLA-ABC Class I Antigen," (clone) "W6/32 is directed against a monomorphic epitope on the 45 kDa polypeptide products of the HLA-A, -B and -C loci. These glycosylated polypeptides are non-covalently associated with B-2 microglobulin, constituting the HLA-A, -B, -C or class I antigen of the human major histocompatibility complex (MHC)" I use a acetone fixation of 4 minutes, then buffer then staining. Hope this helps. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> "Bliven, Laura" <bliven.la...@marshfieldclinic.org> 01/19/09 1:08 PM >>> I'm looking for an MHC Class 1 antibody for staining on frozen muscle sections. Would prefer an unconjugated antibody. Anyone performing this test willing to suggest an antibody and company? Thanks much, Laura Laura Bliven, AAS, HT(ASCP), QIHC Histology Lab/IHC Marshfield Laboratories 1000 N. Oak Ave. Marshfield, WI 54449 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet