Hi all, I would like to label slow and fast cycling progenitor cells using double nucleoside analog labeling.
Can BrdU be distinguished from CldU and/or IdU? Maslov et al (J. Neurosci, 24:1726--1733, 2004) used anti-BrdU antibodies to show both. I assume, one stains one slide of BrdU only treated tissue with anti-BrdU to identify one set of progenitors, and then stains the slides of tissue treated with both (for example) BrdU and CldU and then substracts the first result from the last to get the difference? Or are there specific antibodies to BrdU, CldU and IdU that are available? Can anyone supply a copy of Aten et al (Meth. Cell Biol. 41:317--326, 1994)? Its the reference for the original protocol---unfortunately not available to me. :-( A protocol would be just as welcome. Thanks -- Tyrone Genade http://tgenade.freeshell.org email: tgen...@freeshell.org tel: +27-84-632-1925 (c) ******************************************************************************** "For there is one God, and there is one mediator between God and men, the man Christ Jesus, who gave himself as a ransom for all." 1 Timothy 2:5-6 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet