Hi, I was reading your email of Jan. 15/09 on the subject of "Prion Contamination". In it you had made the following statement, which I was curious as to where this information had come from. "the procedure to inactivate using formic acid is followed before fixation and processing. If the tissue is fixed (formalin or other common fixatives) then you would be actually "fixing" the prion's ability to NOT be inactivated". I had read again the WHO Guidelines on CJD that you had a link to & could find no mention of the fact that the formic acid step for de-activating the prions had to be done before any fixation. The WHO instructions: P. 18 8.2.2 Histopathological examination: states "formic acid treatment consists of placing small pieces of fixed tissue, no more than 4 to 5 mm thick, in 50 to 100 ml of 95% formic acid for an hour". I have been dealing with CJD brains for many years always following the CDC, WHO & Health Canada guidelines but have never read any studies that had indicated that the fixing in formalin made the treatment in formic acid ineffective. If you have any further information I would really appreciate seeing it. Thank you S. Allen sal...@hsc.mb.ca
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