Hello all. I am excited to be a new participant of the Histonet list serve. I
hope that you can provide some assistance in a little problem I am
having.Simply, I wish to photograph blood smear slides (methanol fixed) with
both Hoescht and Giemsa. I want to find items via normal light microscopy from
the Giemsa stain and visualize the Hoescht via our UV imager on the same scope.
The problem I am having is that performing the Giemsa stain first doesn't work
out well because the Hoescht staining solution leeches out the Giemsa and
produces washed out samples with little purple staining. I am trying to do the
Hoescht stain first, but I am afraid that the Giemsa staining conditions might
reduce the ability to resolve the fluorescent signal from the Hoescht
dye.Furthermore, Hoescht binds DNA in the minor groove, while the Giemsa binds
DNA on the phosphate groups. Does any one know about possible inhibition of
binding between these two compounds since they are physically close on the DNA
strand itself?Thank you again for your time and assistance!-Blood Smear-O-Rama
_________________________________________________________________
Windows Live™: E-mail. Chat. Share. Get more ways to connect.
http://windowslive.com/explore?ocid=TXT_TAGLM_WL_t2_allup_explore_012009_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
