Kristen I remember reading an article maybe in the Histology Journal awhile ago. In there it recommended rolling and blotting the tissue with a paper towel to remove excess paraffin and then placing the specimen in a cassette and putting that cassette directly into formalin. I never had an issues with this process.
Sometimes I checked the block to see if I smell xylene. If I do smell xylene that usually means the block needs more time in paraffin. We then usually dropped the blocks in the paraffin tank on the embedding center. They usually stayed there a few hours. Never was any of the fatty tissue compromised from the extended time in paraffin. All of this being said I worked at a very busy placed and we replaced paraffin in our tanks everyday. So paraffin was always clean and never affected by a few blacks place in the embedding tank. Just some thoughts. Best Regards Mari Ann Mailhiot BA HT ASCP Application Specialist/Trainer Leica Microsystems Biosystems Division Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailh...@leica-microsystems.com www.leica-microsystems.com kristen arvidson <arvidsonkristen@ yahoo.com> To Sent by: histonet histonet-bounces@ <histonet@lists.utsouthwestern.edu> lists.utsouthwest cc ern.edu Subject [Histonet] reprocessing 02/03/2009 12:13 PM Please respond to arvidsonkris...@y ahoo.com Hello, What are people doing these days when they have to reprocess fatty tissue? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet