Hi Scott, "slowly freezing on dry ice" may be your problem. In my honest opinion, you need to freeze faster. Try a different method, such as cooled isopentane, either with LN2 (coolest method) or dry ice. You should get better results when you freeze them faster or colder. Good luck, Jo Dee
~~Jo Dee Fish~~ Senior Research Technologist The J. David Gladstone Institutes Co-manager Histology and Microscopy Core Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: jf...@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
