Alexandra I have used it and it's a very good antibody. It works extremely well in both Human and rat tissue. We use at a dilution of 1/2000 (overnight incubation) with EDTA antigen retrieval using a streptavidin detection system.
Cheers Jim Jim Manavis Laboratory Manager Hanson Institute Centre for Neurological Diseases IMVS, Adelaide, SA, 5000 Australia Phone: 61-08-8222-3668 / 0401120697 FAX: 61-08-8222 3392 email: jim.mana...@imvs.sa.gov.au Disclaimer: Not this little black duck! -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Alexandra Meinl Sent: Wednesday, 4 February 2009 9:52 PM To: Histonet Subject: [Histonet] Does anybody use PARP? Hello, Does anybody use Promega's Anti-PARP p85 Fragment on rat FFPE tissue sucessfully? Do you have good results or would you recommend another antibody? Alexandra _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
