Gudrun: I used to predilute the FITC conjugated antibodies and placed the aliquots in a -80ºC freezer until they were going to be used. In that way I could use them until the manufacturer's expiration date. René J.
--- On Thu, 2/5/09, Gudrun Lang <gu.l...@gmx.at> wrote: From: Gudrun Lang <gu.l...@gmx.at> Subject: [Histonet] prediluted Fitc labelled antibodies? To: histonet@lists.utsouthwestern.edu Date: Thursday, February 5, 2009, 11:37 AM Hi all, Has anyone experience with the shelflife of prediluted FITC-labelled antibodies? We use them for direct Immunofluorescence and dilute usually with PBS on the same day of use. With the increasing amount of IF-tests we consider prediluting the antibodies. But I don't know, if there is any negative influence. Thanks in advance Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
