We extended our paraffin infiltration times and that worked out well for us too.
>>> <mari.ann.mailh...@leica-microsystems.com> 2/10/2009 11:10 AM >>> As long as you smell the clearant in the block then putting the blocks in paraffin longer works. I never found it necessary to roll the fat in paper towel and place the specimen back in paraffin. If the block is not fixed ore dehydrated properly and cleared properly putting them in paraffin doesn't work. It all starts at the gross station. Cutting nice thin sections that are fixed for at least 24 hrs. Even before that breast should be bread loafed and placed in formalin and fixed for 24 hrs. Then a section is cut and placed in a cassette and that sits in formalin until it gets placed on the processor. The process of rolling fatty tissue in paper towel and gently pressed allows the fatty specimen to go back in formalin and be reprocessed without taking the specimen back through the xylenes and alcohols to reprocess. Hope this helps. Best regards Mari Ann Mailhiot BA HT ASCP Application Specialist/Trainer Leica Microsystems Biosystems Division Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailh...@leica-microsystems.com www.leica-microsystems.com rmweber...@comcas t.net Sent by: To histonet-bounces@ histonet@lists.utsouthwestern.edu lists.utsouthwest cc ern.edu Subject [Histonet] Breast tissue 02/10/2009 07:49 AM Hello, Do anyone have a better remedy to process fatty breast tissue other than after processing pressing it in paper towels and then putting it back in paraffin for a couple of hours. The pathologist are saying this is taking to long. Thanks, _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet