Piero, Sorry for the delay on the response here. ;-)
We actually now have an IVD rabbit monoclonal CD4 that performs exceptionally well on the Ventana platform (as well as every other platform that it has been tried on). In the past, we tried to develop the other mouse monoclonals (such as the 1F6) ourselves but they did not perform to our expectations. The sensitivity was far too low. These issues were remedied when we developed the rabbit monoclonal version. If you are interested, additional information can be found at: http://www.cellmarque.com/07/p_detail.php?id=2030 Best regards, Isaac Milos Technical Consultant CELL MARQUE CORPORATION 800.665.7284x8991(toll free) 360.981.6505 (mobile) 916.746.8989 (fax) Message: 19 Date: Fri, 7 Nov 2008 20:15:11 +1100 From: "Piero Nelva" <pieronelv...@bigpond.com> Subject: [Histonet] CD4 on Ventana To: <histonet@lists.utsouthwestern.edu> Message-ID: <2d0669f5e5ea4786a17ae07d652ff...@pentium4> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Dear Histonetters I'm having a lot of trouble getting CD4 to stain FFPE tissue using the Ventana Benchmark XT. Any advice about which antibody and protocol suggestions would be greatly appreciated. Piero Nelva Anatomical Pathology Monash Medical Centre Australia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet