I'd say underfixation with 10% NBF. Try extending the time in formalin, before going into decal.
Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Caleri, Kathleen Sent: Tuesday, February 24, 2009 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Giemsa Stain Our lab was using B-5 to fix our bone marrow specimens, and the resulting Giemsa stains were very nice...In order to use the pneumatic tube system at the facility we had to switch to 10% NBF ( we are in the process of choosing a zinc formalin fixative) and now the Giemsas are a funny turquoise, and very pale....has anyone ever encountered this? any ideas?? Regards, Kate Kathleen Caleri, BS, MLT, HT (ASCP) Histology Lab Supervisor Pathology Roswell Park Cancer Institute (716) 845-1329 "the future of mankind lies waiting for those who come to understand their lives and take up their responsibilities to help others" This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet