To the "brainy" histo-people, I have a student doing a cresyl violet stain on some frozen (40um) brain sections. He has been doing this routinely for a couple of months, but his last run looks very odd. There appears to be an area on each section where there is no staining. At first glance I thought it was a fixation problem, but the unstained portion of the tissue is in a different area as you move from slice to slice (these are serial sections), He stains in a Copeland jar and assures me that his stain is well homogenized. Could this be some kind of moisture problem?
Thanks for your thoughts. Katherine Walters Histology Director Central Microscopy Research Facilities 85 Eckstein Medical Research Building University of Iowa Iowa City, Iowa 52242-1101 katherine-walt...@uiowa.edu www.uiowa.edu/~cemrf Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
