Good Morning,
I have an investigator who is bringing me rat brains that have been
injected with gelatin beads. I'm sectioning through the injection
site(s) and she is hoping to see the beads. BUT even though the
injection sites are easy to see there are no gelatin beads present.
My question is, are they being washed out somehow by
fixation/processing or dropping out in the waterbath as she is
telling me? Or are they melting in the paraffin steps (60 degrees)?
If either of these are the case I'm thinking that frozen sections
might be the solution?
Sorry I don't have more info on the beads. I'll try to get that today.
Thanks,
Andi
.....................................................................
: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist University of Arizona :
: (office: AHSC 4212) P.O. Box 245044 :
: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: algra...@u.arizona.edu) :
:...................................................................:
http://www.cba.arizona.edu/histology-lab.html
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