Have you checked out the ton of protocols (including histo) available
at ZFIN online:
http://zfin.org/zf_info/zfbook/zfbk.html
Hi,
I need to work on zebrafish for the first time and would like to get some
general protocols for fixation and IHC staining.
My investigator used 4% PFA for 2 hrs at RT for a whole zebrafish and
sections didn't come out right (falling off & not preserved well).
I am going to increase the time and also try 10% formalin over night. Please
let me know if formalin works ok for IHC later.
Thank you.
Judy
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