Hi to everybody, I have a problem with a recent processing tissue. Normally I process tissue that is very small such as 3mm thick of vessel rings or small cubes of myocardium. Couple of days ago we process a batch of myocardial sections that are really thick. I thought might be thick enough my protocol to perfuse completely. I was wrong it was too thick after the overnight protocol the sections were moist and soft. Of course I didn't even try to embedded them. Question, what can I do now? Can I reprocess them? Once they certain amount of wax in them can I put them again through the alcohols and xylenes and again through paraffin? Or is better just to dry them immersing them in alcohol? I just change the solutions of the processor so before actually going ahead and process them again I preferred to ask since I wouldn't like to get all the solutions dirty with wax with the need to rechange them once again.
I appreciate your answers and help. Armando Tellez atel...@crf.org Pathology Department Cardiovascular Research Foundation Skirball Center for Cardiovascular Research 8 Corporate Drive Orangeburg, New York,10962 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
