Hello:
Recently, I have used Sirius red (using the Kiernan Method found in Histological and Histochemical Methods, 4th Ed. Scion Publishing Ltd. 2008) to localize collagen fibers in livers of mice exposed to carbon tetrachloride over 5 weeks. Unfortunately, I do not have the capability to examine my slides using circular polarizing light microscopy at present, so I cannot determine at the present time whether or not the staining I observe is that of fibrillar collagens or not. I have a question: Is Sirius red (using the above method) able to stain laminin fibers? These data have lead me to develop some exciting, new hypotheses, so I have not yet had the time to research laminin biochemistry to try to answer this question myself. In case you are interested in a bit more background info read the following: My slides show remarkable differences in Sirius red staining depending on from which of my experimental groups the sections came. Specifically, I have noted a peculiarly diffuse Sirius red staining in livers of my carbon tetrachloride-exposed knockout mice while my carbon tetrachloride-exposed wild type mice have 'normal' Sirius red staining. Because of a series of other assays I have performed on these livers (except laminin IHC... I was trying to avoid optimizing a new stain before I had sufficient reason to do so), I suspect that the at much of the diffuse Sirius red staining I have observed in my knockout mice may reflect the presence of more laminin than collagen in the fibrotic livers from knockout mice, and therefore, may explain the odd pattern of ECM deposition. Any thoughts? I thank you, in advance, for your expert advice. -->mtp Michele T. Pritchard, Ph.D. Research Associate-Staff Nagy Laboratory Department of Pathobiology/NE40 Lerner Research Institute Cleveland Clinic 9500 Euclid Avenue Cleveland, OH 44195 phone: 216.444.8613 fax: 216.636.1493 email: prit...@ccf.org Lab location: Lerner Research Institute NE4-214 =================================== P Please consider the environment before printing this e-mail Cleveland Clinic is ranked one of the top hospitals in America by U.S. News & World Report (2008). Visit us online at http://www.clevelandclinic.org for a complete listing of our services, staff and locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
