Sean: Considering that the van't Hoff coefficient determines a metabolic coefficient increase/reduction every 10ºC (Q10) I would increase the processing period one time every 10ºc between the temperature your protocol was designed to work at and the new temperature you are going to use now. Lets say that you fine tuned your protocol for 35 ºC and now you will be working at 4ºC which is a almost 30ºC less = 10ºC x 3 If a given step at 35ºC was set at 1 hour, I would use now 3 hours for the same step. René J.
--- On Wed, 3/11/09, Sean McBride <smcbr...@cs.cmu.edu> wrote: From: Sean McBride <smcbr...@cs.cmu.edu> Subject: [Histonet] Need Advice on Low Temp Tissue Processing To: histonet@lists.utsouthwestern.edu Date: Wednesday, March 11, 2009, 12:30 PM Hi folks, I have a rabbit cranial defect study in which the implant material is sensitive to 100% ethanol at temperatures > 20*C. As I cannot use my my Sakura tissue processor for the specimens (min operating temp=35*C), I plan to process them by hand @ 4*C in a cold room. My intuition tells me that at lower temperatures, the processing times in each solution should be extended by a percentage, but by how much, I have not an idea. Does anyone have any experience or ideas with regards to low temperature tissue processing times? Best regards, ~Sean McBride Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-901-7540 (m) 412-268-8641 (fax) smcbr...@cs.cmu.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet