Did you change paraffins lately? Softer paraffins, ie., lower melting point,
have more compressibility.
Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073
----- Original Message -----
From: "Va Paula Sicurello" <vapat...@yahoo.com>
To: "HistoNet" <histonet@lists.utsouthwestern.edu>
Sent: Wednesday, March 11, 2009 11:29 AM
Subject: [Histonet] Bad sections
Hi Histo netters,
I am am my wit's end. I know how to section but my sections are
compressing like crazy. I've altered my processing protocol thinking that
I was overprocessing my samples.
I've tried different knife angles, different brands of razor blade knives.
It's either me or the microtome.
Info- I process animal tissues with between 30-60 minute steps. Vacuum
paraffins for 30 minutes. Section with Reichert-Jung 2030 set at 4-ish
degrees (a little below the middle line on the knife holder).
Soak blocks on Downey ice blocks, water bath at 44 degrees.
Suggestions?
Waa,
Paula
Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core Research Imaging Center
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397
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