I have cut 20 micron sections of GMA resin using tungsten carbide knives, the cutting is not so difficult (depending on what you are cutting ie calcified bone) as adhering the thick sections to a slide. Thinner sections will stick to + slides a lot better than the thick sections will. So unless they are planning on float staining this will be an obstacle.
Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: Saturday, March 14, 2009 12:48 PM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] 20 micron resin sections Thanks all, This is more or less what i thought, that 20mu sections would prove to be difficult and not all that feasible. So, I wait with bated breath for the protocol from the researcher............ On 3/13/09, Peggy Bisher <mbis...@princeton.edu> wrote: > > One of the labs here use JB4 to section Zebrafish. I sent your question to > them to see if they could help you out. Here is their response: > > The consensus in the lab (Kari and I) is that no, probably not. The size > would probably shred the section and chip it to where they would be uneven, > etc. Probably cryo or vibratome would be best. > > They routinely cut their sections between 2-5 microns. > > Good luck to you! > > Cheers, > > Margaret E. Bisher > Electron Microscopy & Histology Core Facility Manager > Department of Molecular Biology > Princeton University > Moffett Laboratory, Room 113 > Princeton, New Jersey > Office: (609) 258-7026 > Fax: (609) 258-8468 > mbis...@princeton.edu > > > > > > On 3/13/09 3:53 AM, "louise renton" <louise.ren...@gmail.com> wrote: > > > Hi all, > > > > I have a query from a colleague doing research on neuroanatomy as to > whether > > it is possible ( with relative ease) to cut 20mu sections from JB4 resin > > embedded tissue? Apparently these sections ae to be stained and then used > > for stereomicroscopy. My experience is not that extensive to be able to > > answer her, so I would appreciate some advice here > > best regards-- > > Louise Renton > > Bone Research Unit > > University of the Witwatersrand > > Johannesburg > > South Africa > > "There are nights when the wolves are silent and only the moon howls". > > George Carlin > > No trees were killed in the sending of this message. > > However, many electrons were terribly inconvenienced. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet