Hi, I just began to work with the methy methacrylate embedding plastic section. I am facing the difficulities to attach the sample on the glass slide. I have tried using Haupts adhesive coated slides and also the bond-rite slide to attach my 5micron sample. I am doing normal hematoxylin and eosin staining. The section always detach from the glass slide towards the end of the staining. I need to use the concentrated Haupts adhesive coated slide to reduce the chance for the section from lift up from the glass slide. However, I observed the high and dirty background after the staining. I noticed that some users are using Bond-rite slides to attach their samples. I tried this. However, the sections always lifted up towards the end of the staining. I am not too sure whether is my technique that giving me this kind of problem. I prewarmed the Bond-rite slides and put my sections in the 55C waterbath. Then I fished the section using the Bond-rite slide and lay it down on 55C heater. And finally in the oven incubator for more than 18 hours at 55C before staining... I would appreciate if there is any suggestion that can help to attach the section on the glass slide. Thank you very much! Yours sincerely, Ooi Singapore
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