Hello, We have the Thermo Fisher model as well and love it. It does an excellent job on the blocks.in much less time than traditional hand scraping. Karen J Kay, MLT Pathology Supervisor,Chinook Health Laboratory Chinook Regional Hospital,Lethbridge, Alberta, CANADA
-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: March 19, 2009 9:02 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 64, Issue 33 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. equipment purchasing (Patty Dunlop) 2. glycerol buffered antifade media (Neil M. Fournier) 3. Double-stain for immune cell markers (Margaryan, Naira) 4. Paraffin Block triming (Scott) 5. Re: Paraffin Block trimming (Jennifer MacDonald) 6. Re: Paraffin Block triming (Pamela Marcum) 7. RE: RE: Automating muscle and neuro stains (Denise Piontek) 8. Re: Paraffin Block trimming (Angela Bitting) 9. Re: Paraffin Block trimming (Rene J Buesa) 10. RE: Paraffin Block trimming (Pamela Marcum) 11. Re: Paraffin Block triming (Peter Carroll) 12. RE: Paraffin Block trimming (Karin Groeger) 13. RE: Paraffin Block trimming (McMahon, Loralee A) 14. Paraffin Block triming (Paula Pierce) 15. RE: Paraffin Block triming (Bartlett, Jeanine (CDC/CCID/NCZVED)) 16. Re: Paraffin Block trimming (Kelly Boyd) 17. Re: Paraffin Block trimming (godsgal...@aol.com) 18. RE: Paraffin Block trimming (Podawiltz, Thomas) 19. RE: Paraffin Block trimming (Martin, Gary) 20. FW: [Histonet] RE: Automating muscle and neuro stains (Ian Montgomery) 21. Sanderson's RBS Image (Suzanne Bruce) 22. RE: Paraffin Block trimming (Merced Leiker) ---------------------------------------------------------------------- Message: 1 Date: Wed, 18 Mar 2009 10:09:40 -0700 From: Patty Dunlop <pdunlop...@gmail.com> Subject: [Histonet] equipment purchasing To: histonet@lists.utsouthwestern.edu Message-ID: <80ab7bc60903181009o6e178432m42613f7e5cc0e...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hello, Our histo lab is currently looking into buying one or all of the following: cassette labeler, automated stainer (that can also accommodate a few special stains), and automated coverslipper (that uses minimal xylene or no xylene). Can anyone recommend good brands and models that they like as well as what vendors might have good prices? We are also looking into getting the stainer and coverslipper either used or refurbished to cut down on costs. Our lab is small and we do only about 40-50 slides per day on average. We would prefer to have the equipment to be as small as possible. Any suggestions are welcome! Thanks, Patty ------------------------------ Message: 2 Date: Wed, 18 Mar 2009 13:18:23 -0600 From: "Neil M. Fournier" <nfourn...@sasktel.net> Subject: [Histonet] glycerol buffered antifade media To: histonet@lists.utsouthwestern.edu Message-ID: <e742d69a2175489792ebe1cff973c...@neil45eaf11e9e> Content-Type: text/plain; charset=us-ascii Hi Everyone, I have to make up antifade media. The recipe I was given is: 5 ml 0.1 M phosphate buffer, 50 mg p-phenylenediamine, 45 ml glycerol (pH to ~ 10.0 with NaOH) However, I notice that solution became quite a dark yellow when dissolving. Is this normal? It seems to occur every time I try to make it up. Thanks in advance Neil E-mail message checked by Spyware Doctor (6.0.0.386) Database version: 5.11990 http://www.pctools.com/en/spyware-doctor-antivirus/ ------------------------------ Message: 3 Date: Wed, 18 Mar 2009 14:20:34 -0500 From: "Margaryan, Naira" <nmargar...@childrensmemorial.org> Subject: [Histonet] Double-stain for immune cell markers To: <histonet@lists.utsouthwestern.edu> Message-ID: <6a2230bac92e3b4084dae06869b89fb601e6c...@cmhexc01evs.childrensmemorial.org> Content-Type: text/plain; charset="us-ascii" Hi Histofriends, I was requested to make the double-staining for immune cell markers together with another genes (IgG goat) that our lab researching on human tumors xenografted in mouse. Which companies kits you will suggest to use for this experiment (the double-staining) and, what Abs (not mouse and not goat) are the best to represent the immune cell markers. Respectfully, Naira ------------------------------ Message: 4 Date: Wed, 18 Mar 2009 22:19:45 -0600 From: "Scott" <lsc...@sfcn.org> Subject: [Histonet] Paraffin Block triming To: <Histonet@lists.utsouthwestern.edu> Message-ID: <550661ed746d44b48c04db86eb971...@lesliepc> Content-Type: text/plain; charset="iso-8859-1" Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ------------------------------ Message: 5 Date: Wed, 18 Mar 2009 21:26:37 -0700 From: Jennifer MacDonald <jmacdon...@mtsac.edu> Subject: Re: [Histonet] Paraffin Block trimming To: "Scott" <lsc...@sfcn.org> Cc: Histonet@lists.utsouthwestern.edu, histonet-boun...@lists.utsouthwestern.edu Message-ID: <ofedd221f9.07aa17f5-on8825757e.00184c4a-8825757e.0018b...@mtsac.edu> Content-Type: text/plain; charset="US-ASCII" We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" <lsc...@sfcn.org> Sent by: histonet-boun...@lists.utsouthwestern.edu 03/18/2009 09:24 PM To <Histonet@lists.utsouthwestern.edu> cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Thu, 19 Mar 2009 06:34:38 -0400 (EDT) From: Pamela Marcum <pmar...@vet.upenn.edu> Subject: Re: [Histonet] Paraffin Block triming To: Scott <lsc...@sfcn.org> Cc: Histonet@lists.utsouthwestern.edu Message-ID: <1128894179.63154441237458878301.javamail.r...@zm-mbx-modv.zimbra.upenn.edu> Content-Type: text/plain; charset=utf-8 Hi Scott, We have one from Thermo Fisher (Shandon)and love it. It saves time and fingers in cleaning cassettes. I also use it to trim and shape large blocks for sectioning. No shavings no razor blades. Pam Marcum ----- Original Message ----- From: "Scott" <lsc...@sfcn.org> To: Histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 12:19:45 AM GMT -05:00 US/Canada Eastern Subject: [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Thu, 19 Mar 2009 08:28:44 -0400 From: Denise Piontek <dbpion...@hotmail.com> Subject: RE: [Histonet] RE: Automating muscle and neuro stains To: <histonet@lists.utsouthwestern.edu> Message-ID: <col112-w22e7ab3ad4e6cbb1857204ab...@phx.gbl> Content-Type: text/plain; charset="iso-8859-1" I was hoping to determine the level of interest in having muscle and neuro stains automated? I am speaking with a vendor about the desire to automate many of my stains: ATP, NADH, SDH, COX, Acetyl cholinesterase, Luxol Fast Blue, etc..... Would your lab use this technology? Would you do this work in house instead of sending it out if it were automated? How many tests does your lab do per year in this category? Would you prefer this was a feature on your automated special stainer? I will compile the answers. Thanks in advance for your participation. Denise Bland-Piontek, CTBS(AATB)HTL(ASCP)QIHC Technical Director Massachusetts General Hospital _________________________________________________________________ Express your personality in color! Preview and select themes for Hotmail®. http://www.windowslive-hotmail.com/LearnMore/personalize.aspx?ocid=TXT_MSGTX_WL_HM_express_032009#colortheme ------------------------------ Message: 8 Date: Thu, 19 Mar 2009 09:13:46 -0400 From: "Angela Bitting" <akbitt...@geisinger.edu> Subject: Re: [Histonet] Paraffin Block trimming To: <histonet-boun...@lists.utsouthwestern.edu>, "Jennifer MacDonald" <jmacdon...@mtsac.edu>, "Scott" <lsc...@sfcn.org> Cc: Histonet@lists.utsouthwestern.edu Message-ID: <49c20cca.2b7f.00c...@geisinger.edu> Content-Type: text/plain; charset="us-ascii" I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Jennifer MacDonald <jmacdon...@mtsac.edu> 3/19/2009 12:26 AM >>> We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" <lsc...@sfcn.org> Sent by: histonet-boun...@lists.utsouthwestern.edu 03/18/2009 09:24 PM To <Histonet@lists.utsouthwestern.edu> cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ------------------------------ Message: 9 Date: Thu, 19 Mar 2009 06:25:19 -0700 (PDT) From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] Paraffin Block trimming To: histonet-boun...@lists.utsouthwestern.edu, Jennifer MacDonald <jmacdon...@mtsac.edu>, Scott <lsc...@sfcn.org>, Angela Bitting <akbitt...@geisinger.edu> Cc: Histonet@lists.utsouthwestern.edu Message-ID: <767749.47598...@web65708.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 You will have to time worself as I did to find out that waiting fo the paraffin to melt in order to eliminate it takes 2.5 times more time than doing it manually with a pocket knife (as I used). Manually = 32 secs/block vs. 80 secks./block René J. --- On Thu, 3/19/09, Angela Bitting <akbitt...@geisinger.edu> wrote: From: Angela Bitting <akbitt...@geisinger.edu> Subject: Re: [Histonet] Paraffin Block trimming To: histonet-boun...@lists.utsouthwestern.edu, "Jennifer MacDonald" <jmacdon...@mtsac.edu>, "Scott" <lsc...@sfcn.org> Cc: Histonet@lists.utsouthwestern.edu Date: Thursday, March 19, 2009, 9:13 AM I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Jennifer MacDonald <jmacdon...@mtsac.edu> 3/19/2009 12:26 AM >>> We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" <lsc...@sfcn.org> Sent by: histonet-boun...@lists.utsouthwestern.edu 03/18/2009 09:24 PM To <Histonet@lists.utsouthwestern.edu> cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Thu, 19 Mar 2009 09:31:21 -0400 From: "Pamela Marcum" <pmar...@vet.upenn.edu> Subject: RE: [Histonet] Paraffin Block trimming To: "'Angela Bitting'" <akbitt...@geisinger.edu>, <histonet-boun...@lists.utsouthwestern.edu>, "'Jennifer MacDonald'" <jmacdon...@mtsac.edu>, "'Scott'" <lsc...@sfcn.org> Cc: Histonet@lists.utsouthwestern.edu Message-ID: <000c01c9a896$fe832c60$095a5...@vet.upenn.edu> Content-Type: text/plain; charset="US-ASCII" Actually the warm up time is about 3 minutes and after that I can do blocks much faster and cleaner than scraping. I can't imagine it being slower to be honest. We love it as it save a lot of time. Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, March 19, 2009 9:14 AM To: histonet-boun...@lists.utsouthwestern.edu; Jennifer MacDonald; Scott Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Block trimming I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Jennifer MacDonald <jmacdon...@mtsac.edu> 3/19/2009 12:26 AM >>> We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" <lsc...@sfcn.org> Sent by: histonet-boun...@lists.utsouthwestern.edu 03/18/2009 09:24 PM To <Histonet@lists.utsouthwestern.edu> cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ------------------------------ Message: 11 Date: Thu, 19 Mar 2009 09:30:34 -0400 From: Peter Carroll <carro...@umdnj.edu> Subject: Re: [Histonet] Paraffin Block triming To: histonet@lists.utsouthwestern.edu Message-ID: <49c248fa.5060...@umdnj.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed > Does anybody use a paraffin block dewaxer ? Yep, it's called "my own two hands and a metal spatula", ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: > Hi, > Does anybody use a paraffin block dewaxer ? If so does it save > any time, how well does it work? > > Thanks, > > Scott Hendricksen HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ Message: 12 Date: Thu, 19 Mar 2009 06:37:46 -0700 From: "Karin Groeger" <kgroe...@uslabs.net> Subject: RE: [Histonet] Paraffin Block trimming To: "Pamela Marcum" <pmar...@vet.upenn.edu>, "Angela Bitting" <akbitt...@geisinger.edu>, <histonet-boun...@lists.utsouthwestern.edu>, "Jennifer MacDonald" <jmacdon...@mtsac.edu>, "Scott" <lsc...@sfcn.org> Cc: Histonet@lists.utsouthwestern.edu Message-ID: <f5085c791aaf6f47b0170d0b84fabccc0383a...@prodexch01.uslabs.int> Content-Type: text/plain; charset="us-ascii" They work great for us and saves the hand. We use the Shandon Para Trimmer. Our techs love them. Karin Groeger Histology Supervisor US LABS, Irvine,CA 949-450-0145 ext. 649 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Thursday, March 19, 2009 6:31 AM To: 'Angela Bitting'; histonet-boun...@lists.utsouthwestern.edu; 'Jennifer MacDonald'; 'Scott' Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Paraffin Block trimming Actually the warm up time is about 3 minutes and after that I can do blocks much faster and cleaner than scraping. I can't imagine it being slower to be honest. We love it as it save a lot of time. Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, March 19, 2009 9:14 AM To: histonet-boun...@lists.utsouthwestern.edu; Jennifer MacDonald; Scott Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Block trimming I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Jennifer MacDonald <jmacdon...@mtsac.edu> 3/19/2009 12:26 AM >>> We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" <lsc...@sfcn.org> Sent by: histonet-boun...@lists.utsouthwestern.edu 03/18/2009 09:24 PM To <Histonet@lists.utsouthwestern.edu> cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This message, including any attachments, may contain CONFIDENTIAL AND/OR LEGALLY PRIVILEGED information. The information is intended for use by the individual named above and may not be disseminated to any other party without US LABS' written permission. If you are not the intended recipient, or the employee or agent responsible for delivering this information to the intended recipient, you are hereby notified that any dissemination, disclosure, distribution, copying or taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this information in error, please notify US LABS immediately at 1-888-450-0145 attn: Compliance Department to arrange for return of this message including all attachments. ------------------------------ Message: 13 Date: Thu, 19 Mar 2009 09:45:46 -0400 From: "McMahon, Loralee A" <loralee_mcma...@urmc.rochester.edu> Subject: RE: [Histonet] Paraffin Block trimming To: <rjbu...@yahoo.com>, <histonet-boun...@lists.utsouthwestern.edu>, "Jennifer MacDonald" <jmacdon...@mtsac.edu>, "Scott" <lsc...@sfcn.org>, "Angela Bitting" <akbitt...@geisinger.edu> Cc: Histonet@lists.utsouthwestern.edu Message-ID: <2cf6f6b05263ea4ebab07781b51e5db002c94...@e2k3ms1.urmc-sh.rochester.edu> Content-Type: text/plain; charset="iso-8859-1" In my experience the paraffin block trimmer is quicker and easier on your hands than the old manual scraping method. Loralee McMahon, HTL (ASCP) ICC Supervisor University of Rochester Department of Pathology (585) 275-7210 ________________________________ From: histonet-boun...@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Thu 3/19/2009 9:25 AM To: histonet-boun...@lists.utsouthwestern.edu; Jennifer MacDonald; Scott; Angela Bitting Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Block trimming You will have to time worself as I did to find out that waiting fo the paraffin to melt in order to eliminate it takes 2.5 times more time than doing it manually with a pocket knife (as I used). Manually = 32 secs/block vs. 80 secks./block René J. --- On Thu, 3/19/09, Angela Bitting <akbitt...@geisinger.edu> wrote: From: Angela Bitting <akbitt...@geisinger.edu> Subject: Re: [Histonet] Paraffin Block trimming To: histonet-boun...@lists.utsouthwestern.edu, "Jennifer MacDonald" <jmacdon...@mtsac.edu>, "Scott" <lsc...@sfcn.org> Cc: Histonet@lists.utsouthwestern.edu Date: Thursday, March 19, 2009, 9:13 AM I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Jennifer MacDonald <jmacdon...@mtsac.edu> 3/19/2009 12:26 AM >>> We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" <lsc...@sfcn.org> Sent by: histonet-boun...@lists.utsouthwestern.edu 03/18/2009 09:24 PM To <Histonet@lists.utsouthwestern.edu> cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Thu, 19 Mar 2009 06:56:46 -0700 (PDT) From: Paula Pierce <cont...@excaliburpathology.com> Subject: [Histonet] Paraffin Block triming To: Histonet <histonet@lists.utsouthwestern.edu> Message-ID: <769731.2958...@web1107.biz.mail.sk1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I do scrape using the handle end of the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP ________________________________ From: Peter Carroll <carro...@umdnj.edu> To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming > Does anybody use a paraffin block dewaxer ? Yep, it's called "my own two hands and a metal spatula", ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: > Hi, > Does anybody use a paraffin block dewaxer ? If so does it save any >time, how well does it work? > > Thanks, > > Scott Hendricksen HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Thu, 19 Mar 2009 09:58:25 -0400 From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" <j...@cdc.gov> Subject: RE: [Histonet] Paraffin Block triming To: "Paula Pierce" <cont...@excaliburpathology.com>, Histonet <histonet@lists.utsouthwestern.edu> Message-ID: <9a16cb5d55fc1648adf11b63e72a1be1bf0...@lta3vs011.ees.hhs.gov> Content-Type: text/plain; charset=iso-8859-1 I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I do scrape using the handle end of the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP ________________________________ From: Peter Carroll <carro...@umdnj.edu> To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming > Does anybody use a paraffin block dewaxer ? Yep, it's called "my own two hands and a metal spatula", ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: > Hi, > Does anybody use a paraffin block dewaxer ? If so does it save any >time, how well does it work? > > Thanks, > > Scott Hendricksen HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Thu, 19 Mar 2009 07:03:08 -0700 (PDT) From: Kelly Boyd <kdboydhi...@yahoo.com> Subject: Re: [Histonet] Paraffin Block trimming To: histonet-boun...@lists.utsouthwestern.edu, Jennifer MacDonald <jmacdon...@mtsac.edu>, Scott <lsc...@sfcn.org>, Angela Bitting <akbitt...@geisinger.edu>, rjbu...@yahoo.com Cc: Histonet@lists.utsouthwestern.edu Message-ID: <32862.36509...@web58608.mail.re3.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 My techs use our Para Trimmer from Thermo Scientific Shandon. It will save your hands, but it takes a lot more time than the old fashion hand scraping. I prefer to hand scrape. I can scrape at least twice as many by hand than with the para trimmer. Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com Tele (252)-830-6866 Cell (252)-943-9527 Fax (252)-830-0032 --- On Thu, 3/19/09, Rene J Buesa <rjbu...@yahoo.com> wrote: From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] Paraffin Block trimming To: histonet-boun...@lists.utsouthwestern.edu, "Jennifer MacDonald" <jmacdon...@mtsac.edu>, "Scott" <lsc...@sfcn.org>, "Angela Bitting" <akbitt...@geisinger.edu> Cc: Histonet@lists.utsouthwestern.edu Date: Thursday, March 19, 2009, 9:25 AM You will have to time worself as I did to find out that waiting fo the paraffin to melt in order to eliminate it takes 2.5 times more time than doing it manually with a pocket knife (as I used). Manually = 32 secs/block vs. 80 secks./block René J. --- On Thu, 3/19/09, Angela Bitting <akbitt...@geisinger.edu> wrote: From: Angela Bitting <akbitt...@geisinger.edu> Subject: Re: [Histonet] Paraffin Block trimming To: histonet-boun...@lists.utsouthwestern.edu, "Jennifer MacDonald" <jmacdon...@mtsac.edu>, "Scott" <lsc...@sfcn.org> Cc: Histonet@lists.utsouthwestern.edu Date: Thursday, March 19, 2009, 9:13 AM I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Jennifer MacDonald <jmacdon...@mtsac.edu> 3/19/2009 12:26 AM >>> We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" <lsc...@sfcn.org> Sent by: histonet-boun...@lists.utsouthwestern.edu 03/18/2009 09:24 PM To <Histonet@lists.utsouthwestern.edu> cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Thu, 19 Mar 2009 10:08:40 -0400 From: godsgal...@aol.com Subject: Re: [Histonet] Paraffin Block trimming To: kgroe...@uslabs.net, pmar...@vet.upenn.edu, akbitt...@geisinger.edu, histonet-boun...@lists.utsouthwestern.edu, jmacdon...@mtsac.edu, lsc...@sfcn.org Cc: Histonet@lists.utsouthwestern.edu Message-ID: <8cb76b3572a802b-1288-3...@webmail-md03.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" I agree that this is quicker than manually---you can do 4?or 5 blocks at a time. -----Original Message----- From: Karin Groeger <kgroe...@uslabs.net> To: Pamela Marcum <pmar...@vet.upenn.edu>; Angela Bitting <akbitt...@geisinger.edu>; histonet-boun...@lists.utsouthwestern.edu; Jennifer MacDonald <jmacdon...@mtsac.edu>; Scott <lsc...@sfcn.org> Cc: Histonet@lists.utsouthwestern.edu Sent: Thu, 19 Mar 2009 9:37 am Subject: RE: [Histonet] Paraffin Block trimming They work great for us and saves the hand. We use the Shandon Para Trimmer. Our techs love them. Karin Groeger Histology Supervisor US LABS, Irvine,CA 949-450-0145 ext. 649 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Thursday, March 19, 2009 6:31 AM To: 'Angela Bitting'; histonet-boun...@lists.utsouthwestern.edu; 'Jennifer MacDonald'; 'Scott' Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Paraffin Block trimming Actually the warm up time is about 3 minutes and after that I can do blocks much faster and cleaner than scraping. I can't imagine it being slower to be honest. We love it as it save a lot of time. Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, March 19, 2009 9:14 AM To: histonet-boun...@lists.utsouthwestern.edu; Jennifer MacDonald; Scott Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Block trimming I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Jennifer MacDonald <jmacdon...@mtsac.edu> 3/19/2009 12:26 AM >>> We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" <lsc...@sfcn.org> Sent by: histonet-boun...@lists.utsouthwestern.edu 03/18/2009 09:24 PM To <Histonet@lists.utsouthwestern.edu> cc Subject [Histonet] Paraffin Block triming Hi, Does anyb ody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This message, including any attachments, may contain CONFIDENTIAL AND/OR LEGALLY PRIVILEGED information. The information is intended for use by the individual named above and may not be disseminated to any other party without US LABS' written permission. If you are not the intended recipient, or the employee or agent responsible for delivering this information to the intended recipient, you are hereby notified that any dissemination, disclosure, distribution, copying or taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this information in error, please notify US LABS immediately at 1-888-450-0145 attn: Compliance Department to arrange for return of this me ssage including all attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Thu, 19 Mar 2009 10:24:00 -0400 From: "Podawiltz, Thomas" <tpodawi...@lrgh.org> Subject: RE: [Histonet] Paraffin Block trimming To: Kelly Boyd <kdboydhi...@yahoo.com>, "histonet-boun...@lists.utsouthwestern.edu" <histonet-boun...@lists.utsouthwestern.edu>, Jennifer MacDonald <jmacdon...@mtsac.edu>, Scott <lsc...@sfcn.org>, Angela Bitting <akbitt...@geisinger.edu>, "rjbu...@yahoo.com" <rjbu...@yahoo.com> Cc: "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> Message-ID: <38667e7fb77ecd4e91bfaeb8d98638631d33b5b...@lrghexvs1.practice.lrgh.org> Content-Type: text/plain; charset="iso-8859-1" We have one timmer. I don't use it, I hand trim simply because I am quicker with it. The other techs use the timmer to save their hands. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kelly Boyd [kdboydhi...@yahoo.com] Sent: Thursday, March 19, 2009 10:03 AM To: histonet-boun...@lists.utsouthwestern.edu; Jennifer MacDonald; Scott; Angela Bitting; rjbu...@yahoo.com Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Block trimming My techs use our Para Trimmer from Thermo Scientific Shandon. It will save your hands, but it takes a lot more time than the old fashion hand scraping. I prefer to hand scrape. I can scrape at least twice as many by hand than with the para trimmer. Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com Tele (252)-830-6866 Cell (252)-943-9527 Fax (252)-830-0032 --- On Thu, 3/19/09, Rene J Buesa <rjbu...@yahoo.com> wrote: From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] Paraffin Block trimming To: histonet-boun...@lists.utsouthwestern.edu, "Jennifer MacDonald" <jmacdon...@mtsac.edu>, "Scott" <lsc...@sfcn.org>, "Angela Bitting" <akbitt...@geisinger.edu> Cc: Histonet@lists.utsouthwestern.edu Date: Thursday, March 19, 2009, 9:25 AM You will have to time worself as I did to find out that waiting fo the paraffin to melt in order to eliminate it takes 2.5 times more time than doing it manually with a pocket knife (as I used). Manually = 32 secs/block vs. 80 secks./block René J. --- On Thu, 3/19/09, Angela Bitting <akbitt...@geisinger.edu> wrote: From: Angela Bitting <akbitt...@geisinger.edu> Subject: Re: [Histonet] Paraffin Block trimming To: histonet-boun...@lists.utsouthwestern.edu, "Jennifer MacDonald" <jmacdon...@mtsac.edu>, "Scott" <lsc...@sfcn.org> Cc: Histonet@lists.utsouthwestern.edu Date: Thursday, March 19, 2009, 9:13 AM I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Jennifer MacDonald <jmacdon...@mtsac.edu> 3/19/2009 12:26 AM >>> We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald "Scott" <lsc...@sfcn.org> Sent by: histonet-boun...@lists.utsouthwestern.edu 03/18/2009 09:24 PM To <Histonet@lists.utsouthwestern.edu> cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ------------------------------ Message: 19 Date: Thu, 19 Mar 2009 07:37:13 -0700 From: "Martin, Gary" <gmar...@marshallmedical.org> Subject: RE: [Histonet] Paraffin Block trimming To: "Bartlett, Jeanine \(CDC/CCID/NCZVED\)" <j...@cdc.gov>, "Paula Pierce" <cont...@excaliburpathology.com>, "Histonet" <histonet@lists.utsouthwestern.edu> Message-ID: <6ed9d4252f278841a0593d3d788af24c04d28...@mailsvr.marshmed.local> Content-Type: text/plain; charset="iso-8859-1" No you're not the only one ... I was wondering the same thing ... why all the scraping. It seems to me that clean embedding does the trick with a few exceptions. G -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I do scrape using the handle end of the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP ________________________________ From: Peter Carroll <carro...@umdnj.edu> To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming > Does anybody use a paraffin block dewaxer ? Yep, it's called "my own two hands and a metal spatula", ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: > Hi, > Does anybody use a paraffin block dewaxer ? If so does it save any >time, how well does it work? > > Thanks, > > Scott Hendricksen HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Thu, 19 Mar 2009 14:38:58 -0000 From: "Ian Montgomery" <ian.montgom...@bio.gla.ac.uk> Subject: FW: [Histonet] RE: Automating muscle and neuro stains To: <histonet@lists.utsouthwestern.edu> Message-ID: <57d70b824966452fa0d788fd34277...@ibls.gla.ac.uk> Content-Type: text/plain; charset="us-ascii" Have I died and gone to histologists heaven? Automate an ATPase, now there would be something special. No more crying and wailing over failed or partially successful acid reversal. I'll have to retreat to a darkened room, I'm traumatised. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. Subject: RE: [Histonet] RE: Automating muscle and neuro stains I was hoping to determine the level of interest in having muscle and neuro stains automated? I am speaking with a vendor about the desire to automate many of my stains: ATP, NADH, SDH, COX, Acetyl cholinesterase, Luxol Fast Blue, etc..... Would your lab use this technology? Would you do this work in house instead of sending it out if it were automated? How many tests does your lab do per year in this category? Would you prefer this was a feature on your automated special stainer? I will compile the answers. Thanks in advance for your participation. Denise Bland-Piontek, CTBS(AATB)HTL(ASCP)QIHC Technical Director Massachusetts General Hospital _________________________________________________________________ Express your personality in color! Preview and select themes for HotmailR. http://www.windowslive-hotmail.com/LearnMore/personalize.aspx?ocid=TXT_MSGTX _WL_HM_express_032009#colortheme____________________________________________ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Thu, 19 Mar 2009 10:55:20 -0400 From: "Suzanne Bruce" <sbr...@vetpathservicesinc.com> Subject: [Histonet] Sanderson's RBS Image To: "histonet" <histonet@lists.utsouthwestern.edu> Message-ID: <26db1fdfbf9ee14ab3aacc873519a4a2855...@vpss1.vetpathservicesinc.local> Content-Type: text/plain; charset="iso-8859-1" Hi, Does anyone have a picture of what a slide stained w/Sanderson's Rapid Bone should look like? Thanks in advance, Suzanne _______________________________ Suzanne Bruce, R.V.T. Histologist & Necropsy Coordinator <https://69.61.197.115/exchweb/bin/redir.asp?URL=http://www.vetpathservicesinc.com/> ------------------------------ Message: 22 Date: Thu, 19 Mar 2009 10:55:36 -0400 From: Merced Leiker <lei...@buffalo.edu> Subject: RE: [Histonet] Paraffin Block trimming To: "Martin, Gary" <gmar...@marshallmedical.org>, "Bartlett, Jeanine (CDC/CCID/NCZVED)" <j...@cdc.gov>, Paula Pierce <cont...@excaliburpathology.com>, Histonet <histonet@lists.utsouthwestern.edu> Message-ID: <1e3c94c039a525739e077...@bchwxp2702.ad.med.buffalo.edu> Content-Type: text/plain; charset=iso-8859-1; format=flowed Now I'm interested in how you embed so as to not have to scrape...if i don't add enough wax (enough to end up rising around the edges of the cassette, hence the scraping later), i don't get a secure hold of the block to the cassette... ML --On Thursday, March 19, 2009 7:37 AM -0700 "Martin, Gary" <gmar...@marshallmedical.org> wrote: > No you're not the only one ... I was wondering the same thing ... why > all the scraping. It seems to me that clean embedding does the trick > with a few exceptions. G > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of > Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 > 6:58 AM > To: Paula Pierce; Histonet > Subject: RE: [Histonet] Paraffin Block triming > > I was wondering if I was the only one out there that rarely has to > scrape a block. > > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartl...@cdc.hhs.gov > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula > Pierce Sent: Thursday, March 19, 2009 9:57 AM > To: Histonet > Subject: [Histonet] Paraffin Block triming > > I try to embed so as to have a minimal amount of paraffin to scrape > from the blocks. ;) > > But, I do scrape using the handle end of the same forceps I use to > pick up the ribbon and tease the sections. No sharp edge. No > electricity. > > PKP > > > > > > > ________________________________ > From: Peter Carroll <carro...@umdnj.edu> > To: histonet@lists.utsouthwestern.edu > Sent: Thursday, March 19, 2009 8:30:34 AM > Subject: Re: [Histonet] Paraffin Block triming > >> Does anybody use a paraffin block dewaxer ? > > Yep, it's called "my own two hands and a metal spatula", ha ha :) I > find that it's not only very quick, but quite accurate... > > > > Scott wrote: >> Hi, >> Does anybody use a paraffin block dewaxer ? If so does it save >> any time, how well does it work? >> >> Thanks, >> >> Scott Hendricksen HT (ASCP) >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 64, Issue 33 **************************************** This communication is intended for the use of the recipient to which it is addressed, and may contain confidential, personal and or privileged information. Please contact us immediately if you are not the intended recipient. Do not copy, distribute or take action relying on it. Any communication received in error, or subsequent reply, should be deleted or destroyed. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet