We have used a variety of cassettes and paraffin over the years...same result.
Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 [email protected] -----Original Message----- From: Merced Leiker [mailto:[email protected]] Sent: Thursday, March 19, 2009 11:16 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Martin, Gary; Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block trimming hmmm...I do too...Fisher 15-182-505B HistoPrep Stainless-Steel Base Molds...maybe it's the cassette design or type of wax used? (VWR 15147-839 POLYFIN, VWR 87002-362 HISTOSETTE Cassettes, Simport Plastics Biopsy Cassettes) --On Thursday, March 19, 2009 10:57 AM -0400 "Bartlett, Jeanine (CDC/CCID/NCZVED)" <[email protected]> wrote: > Don't know what to say.........I fill mine up and as long as I don't get > to rough with it (bump it or something where it sloshes) I don't have any > excess paraffin around the edges. I use metal base molds. > > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > [email protected] > > > -----Original Message----- > From: Merced Leiker [mailto:[email protected]] > Sent: Thursday, March 19, 2009 10:56 AM > To: Martin, Gary; Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; > Histonet Subject: RE: [Histonet] Paraffin Block trimming > > Now I'm interested in how you embed so as to not have to scrape...if i > don't add enough wax (enough to end up rising around the edges of the > cassette, hence the scraping later), i don't get a secure hold of the > block to the cassette... > > ML > > --On Thursday, March 19, 2009 7:37 AM -0700 "Martin, Gary" > <[email protected]> wrote: > >> No you're not the only one ... I was wondering the same thing ... why all >> the scraping. It seems to me that clean embedding does the trick with a >> few exceptions. G >> >> -----Original Message----- >> From: [email protected] >> [mailto:[email protected]] On Behalf Of Bartlett, >> Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM >> To: Paula Pierce; Histonet >> Subject: RE: [Histonet] Paraffin Block triming >> >> I was wondering if I was the only one out there that rarely has to scrape >> a block. >> >> >> Jeanine Bartlett >> Infectious Diseases Pathology Branch >> (404) 639-3590 >> [email protected] >> >> >> -----Original Message----- >> From: [email protected] >> [mailto:[email protected]] On Behalf Of Paula >> Pierce Sent: Thursday, March 19, 2009 9:57 AM >> To: Histonet >> Subject: [Histonet] Paraffin Block triming >> >> I try to embed so as to have a minimal amount of paraffin to scrape from >> the blocks. ;) >> >> But, I do scrape using the handle end of the same forceps I use to pick >> up the ribbon and tease the sections. No sharp edge. No electricity. >> >> PKP >> >> >> >> >> >> >> ________________________________ >> From: Peter Carroll <[email protected]> >> To: [email protected] >> Sent: Thursday, March 19, 2009 8:30:34 AM >> Subject: Re: [Histonet] Paraffin Block triming >> >>> Does anybody use a paraffin block dewaxer ? >> >> Yep, it's called "my own two hands and a metal spatula", ha ha :) I find >> that it's not only very quick, but quite accurate... >> >> >> >> Scott wrote: >>> Hi, >>> Does anybody use a paraffin block dewaxer ? If so does it save any >>> time, how well does it work? >>> >>> Thanks, >>> >>> Scott Hendricksen HT (ASCP) >>> _______________________________________________ >>> Histonet mailing list >>> [email protected] >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >>> >> >> >> _______________________________________________ >> Histonet mailing list >> [email protected] >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> [email protected] >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> [email protected] >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> [email protected] >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > Merced M Leiker > Research Technician II > 354 Biomedical Research Building > School of Medicine and Biomedical Sciences > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 > Ph: (716) 829-6033 > Fx: (716) 829-2725 > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
