I think it depends on whether you place your cassette bottom side down (slotted side close to the tissue) or bottom side up (open side close to the tissue) when embedding.
The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 10:07 AM To: hymclab; Doug Showers; Histonet Subject: RE: [Histonet] Paraffin Block triming I also use mine warm........ Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of hymclab Sent: Thursday, March 19, 2009 1:03 PM To: 'Doug Showers'; Histonet Subject: RE: [Histonet] Paraffin Block triming I disagree. I take my molds right out of the holding chamber and use warm and have hardly any leakage and don't have to hardly scrape. However, the other techs do have leakage and have to scrape alot off. I think it is just in the embedders technique. Dawn -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Doug Showers Sent: Thursday, March 19, 2009 11:37 AM To: Histonet Subject: RE: [Histonet] Paraffin Block triming I think the amount of excess paraffin depends on the temperature of the mold when you are embedding. I like my molds "cold" at room temperature and don't have much leakage whereas techs who keep the molds "hot" have a higher amount of leakage around the edges. Doug Showers, MS, HT Histology Manager ProPath 8267 Elmbrook Dr. Suite 100 Dallas, TX 75247 To learn more about ProPath, please visit http://www.ProPathLab.com -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shakun Aswani Sent: Thursday, March 19, 2009 11:03 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming Jeanine, We had the best Chief instructor Billie Swisher. I am so very thankful that I got the training from that school. I do miss you guys Shakun -----Original Message----- From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:j...@cdc.gov] Sent: Thursday, March 19, 2009 8:45 AM To: Shakun Aswani; Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming Of course Shakun....you trained me! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -----Original Message----- From: Shakun Aswani [mailto:shakun.asw...@acologix.com] Sent: Thursday, March 19, 2009 11:09 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I am also one of that -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I do scrape using the handle end of the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP ________________________________ From: Peter Carroll <carro...@umdnj.edu> To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming > Does anybody use a paraffin block dewaxer ? Yep, it's called "my own two hands and a metal spatula", ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: > Hi, > Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? > > Thanks, > > Scott Hendricksen HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. 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