We don't let our unfixed sections air-dry (room temp) long before fixing
them - a few minutes? For some people here I let them sit a few minutes
then just store them in the -80 for up to a weeks weeks before they fix
them and commence with their staining procedure. If the animals were
perfusion-fixed maybe they can air-dry longer. I've heard everything from 5
min to overnight. At room temperature.
Just my input.
--On Tuesday, March 24, 2009 12:17 PM -0400 "Vanessa J. Phelan"
<vjp2...@columbia.edu> wrote:
Hi everyone,
When carrying out IF on frozen sections how long after the cutting the
sections should you leave the slides to dry and where before starting the
staining procedure?
Thanks a mill,
V
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