4-12 hours dependent on sample size (a nickel if a good reference)...volume 1:20
-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, March 25, 2009 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 64, Issue 44 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: questions re: fixing in general and Histochoice in particular (Geoff McAuliffe) 2. Information Systems: Specimen Tracking & MiddleWare (Michael Mihalik) 3. RE: Current books for Histotechnology (Smith, Allen) 4. Part Time Histotechnologist (Chicago, IL) (Crawford, Jennifer) 5. RE: Current books for Histotechnology (Smith, Allen) 6. RE: Current books for Histotechnology (Smith, Allen) 7. formaldehyde neutralizers (Robert Richmond) 8. Re: formaldehyde neutralizers (Rene J Buesa) 9. Histology Supervisor, Permanent Job, FREE MEDICAL BENEFITS! (Alyssa Peterson) 10. Frozen section (Martin, Gary) 11. RE: Frozen section (McNabola, Angela) 12. Re: Current books for Histotechnology (Geoff McAuliffe) 13. RE: Frozen section (Weems, Joyce) 14. RNA and DNA yields from Laser Microdissection (Barone, Carol ) 15. Re: RNA and DNA yields from Laser Microdissection (ne...@staff.uni-marburg.de) ---------------------------------------------------------------------- Message: 1 Date: Wed, 25 Mar 2009 09:35:32 -0400 From: Geoff McAuliffe <mcaul...@umdnj.edu> Subject: Re: [Histonet] questions re: fixing in general and Histochoice in particular To: Jacqui Detmar <det...@lunenfeld.ca> Cc: histonet@lists.utsouthwestern.edu Message-ID: <49ca3324.1070...@umdnj.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Jacqui Detmar wrote: > Hi all. Having a bit of an internal debate here, so I would like to get > the opinions of some of you in Histoland, please. Here are the > questions: > > > > 1. When fixing with 10% NBF, for how long should you fix and what > volume ratio of fixative:tissue should you use? > 48 hours is a minimum according to R.D. Lillie, one week is better. Really! Thickness of the tissue does not matter, formalin fixes slowly. Of course, if you are doing immuno you may need to sacrifice some fixation to retain antigenicity. Depends on the antigen. 10X the volume of the tissue. > 2. At what temperature should one be fixing tissues? > Room temperature. There is more than ample scientific evidence that fixing cold just slows down the already slow fixation process. > > > Regarding Histochoice: > I never use proprietary fixes, you don't know what is in them and the manufacturer can change the formulation any time he/she wants. > > > 1. For how long should you fix the tissue? > > 2. What volume ratio of fixative:tissue should you use? > > 3. How long can you store Histochoice-fixed tissues in 70% > ethanol? > > > > I think that's about it. Thanks in advance, > > > > Jacqui > > > > Jacqui Detmar, Post-doctoral Fellow > > Samuel Lunenfeld Research Institute > > Mount Sinai Hospital > > 25 Orde Street, room 6-1001AJ > > Toronto, ON M5T 3H7 > > > > Email: det...@lunenfeld.ca > > Phone: 416-586-4800 x5607 > > Fax: 416-586-5993 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Geoff -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ********************************************** ------------------------------ Message: 2 Date: Wed, 25 Mar 2009 08:46:11 -0500 From: "Michael Mihalik" <m...@pathview.com> Subject: [Histonet] Information Systems: Specimen Tracking & MiddleWare To: <histonet@lists.utsouthwestern.edu> Message-ID: <00c001c9ad50$13204280$3960c7...@com> Content-Type: text/plain; charset="US-ASCII" Good morning, I was just at the Lab Infotech Summit in Las Vegas last week where the subject of the conference was informatics in Anatomic and Clinical Pathology. Along with the usual seminars were the usual vendors in the exhibitor's hall demonstrating and talking about their products and services. As one of those vendors, I had the opportunity to talk to a few people and a general trend appeared to merge -- one which I would like to dispel, if possible. I'd like to make sure that everyone is aware that you do NOT have to have middleware in order to have bar coded cassettes, slides, etc., and you do NOT have to have middleware in order to have specimen/material tracking. Let me explain. If, on the one hand, you are quite content with your current information system and you simply wish to add barcodes and specimen tracking and you do not want to work with your information system vendor because either they don't have this capability or for some other reason, then YES, middleware is a viable alternative. On the other hand, if you are planning to purchase a new Information System for your laboratory, then by all means, DEMAND of your new vendor, the ability to have barcoded everything and to have specimen tracking built into your new information system. There are lots of good reasons to have all this capability in your information system and not in some middleware product. I'd be happy to discuss the reasons for my statements, but I've taken up enough of everyone's time. If you'd like to hear more, then please, just ask. I just thought everyone should know... Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 ------------------------------ Message: 3 Date: Wed, 25 Mar 2009 09:46:22 -0400 From: "Smith, Allen" <asm...@mail.barry.edu> Subject: RE: [Histonet] Current books for Histotechnology To: 'Jennifer MacDonald' <jmacdon...@mtsac.edu> Cc: "'Histonet@lists.utsouthwestern.edu'" <Histonet@lists.utsouthwestern.edu> Message-ID: <e4132130ac2f764d8c173c5400d53042a220d6d...@exchsrv02.barrynet.barry.edu> Content-Type: text/plain; charset="us-ascii" -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Friday, March 20, 2009 4:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Current books for Histotechnology Our library has funds available to purchase books for the Histotechnology program. The problem is that we need current books. We have the latest Bancroft and Gamble. Any other suggestions for books that are newer than 2000? I have suggested John Kiernan's latest. By the way I did find a copy of Sheehan for $2,400!! Jennifer MacDonald Education Coordinator, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdon...@mtsac.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Wed, 25 Mar 2009 09:53:21 -0400 From: "Crawford, Jennifer" <jcraw...@aerotek.com> Subject: [Histonet] Part Time Histotechnologist (Chicago, IL) To: <histonet@lists.utsouthwestern.edu> Message-ID: <571a823e0300f549be86279a872395770283f...@ag00-exmbx04.allegisgroup.com> Content-Type: text/plain; charset="us-ascii" Good morning! I currently have a part time Histotechnologist position available in the south suburbs of Chicago. The shifts are 10 hour days but only 1-3 days per week are required with occasional Saturdays. ASCP certification is required. Please contact me directly at jcraw...@aerotek.com if you or someone you know is interested! Best regards, Jen Jen Crawford, CIR Scientific Recruiter Aerotek Scientific Staffing Phone: 847.221.1358 Fax: 847.303.2370 www.aerotek.com <http://www.aerotek.com> Please do not keep me a secret...a referral is the best compliment that I can receive! ____________________________________________________________________________________________________ This electronic mail (including any attachments) may contain information that is privileged, confidential, and/or otherwise protected from disclosure to anyone other than its intended recipient(s). Any dissemination or use of this electronic email or its contents (including any attachments) by persons other than the intended recipient(s) is strictly prohibited. If you have received this message in error, please notify us immediately by reply email so that we may correct our internal records. Please then delete the original message (including any attachments) in its entirety. Thank you. ------------------------------ Message: 5 Date: Wed, 25 Mar 2009 09:55:51 -0400 From: "Smith, Allen" <asm...@mail.barry.edu> Subject: RE: [Histonet] Current books for Histotechnology To: 'Jennifer MacDonald' <jmacdon...@mtsac.edu> Cc: "'Histonet@lists.utsouthwestern.edu'" <Histonet@lists.utsouthwestern.edu> Message-ID: <e4132130ac2f764d8c173c5400d53042a220d6d...@exchsrv02.barrynet.barry.edu> Content-Type: text/plain; charset="us-ascii" Definitely get the 4th edition of Kiernan. Also get the latest edition of Polak and van Noorden's INTRODUCTION TO IMMUNOCYTOCHEMISTRY. If you don't already have them, used second or third editions of Lillie's HISTOPATHOLOGIC TECHNIC AND PRACTICAL HISTOCHEMISTRY and Pierce's HISTOCHEMISTRY, THEORETICAL AND APPLIED are surprisingly useful. -Allen A. Smith,Ph.D. Barry University School of Podiatric Medicine Miami Shores, Florida -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Friday, March 20, 2009 4:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Current books for Histotechnology Our library has funds available to purchase books for the Histotechnology program. The problem is that we need current books. We have the latest Bancroft and Gamble. Any other suggestions for books that are newer than 2000? I have suggested John Kiernan's latest. By the way I did find a copy of Sheehan for $2,400!! Jennifer MacDonald Education Coordinator, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdon...@mtsac.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Wed, 25 Mar 2009 10:01:53 -0400 From: "Smith, Allen" <asm...@mail.barry.edu> Subject: RE: [Histonet] Current books for Histotechnology To: 'Ingles Claire ' <cing...@uwhealth.org> Cc: "'Histonet@lists.utsouthwestern.edu'" <Histonet@lists.utsouthwestern.edu> Message-ID: <e4132130ac2f764d8c173c5400d53042a220d6d...@exchsrv02.barrynet.barry.edu> Content-Type: text/plain; charset="us-ascii" For an atlas, Ross and Pawlina's HISTOLOGY, Wheater's FUNCTIONAL HISTOLOGY, or Gartner and Hiatt's COLOR ATLAS OF HISTOLOGY are all good. They are also more accurate than DiFiore. -Allen a. Smith,Ph.D. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Monday, March 23, 2009 11:55 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Current books for Histotechnology I would strongly recommend di Fiore's Atlas of Histology by Victor P. Eroschenko. I don't know what edition its in now. I used it when I went through my program. It is great for microscopic anatomy, especially when combined with actual slide viewing. Claire ________________________________ From: histonet-boun...@lists.utsouthwestern.edu on behalf of Jennifer MacDonald Sent: Fri 3/20/2009 3:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Current books for Histotechnology Our library has funds available to purchase books for the Histotechnology program. The problem is that we need current books. We have the latest Bancroft and Gamble. Any other suggestions for books that are newer than 2000? I have suggested John Kiernan's latest. By the way I did find a copy of Sheehan for $2,400!! Jennifer MacDonald Education Coordinator, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdon...@mtsac.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Wed, 25 Mar 2009 10:06:03 -0400 From: Robert Richmond <rsrichm...@aol.com> Subject: [Histonet] formaldehyde neutralizers To: histonet@lists.utsouthwestern.edu Message-ID: <abea52a60903250706p50a524c0ic88b4c1f734e5...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Thanks, Tony Henwood! Your explanation of how to neutralize formaldehyde with ammonia is the only clear explanation of formaldehyde neutralization I've ever read. One more question: how does neutralization with sodium bisulfite work? What are the advantages and disadvantages of the two methods? Bob Richmond Samurai Pathologist Knoxville, Tennessee ------------------------------ Message: 8 Date: Wed, 25 Mar 2009 07:38:45 -0700 (PDT) From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] formaldehyde neutralizers To: histonet@lists.utsouthwestern.edu, Robert Richmond <rsrichm...@aol.com> Message-ID: <925805.52259...@web65705.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Me too! René J. --- On Wed, 3/25/09, Robert Richmond <rsrichm...@aol.com> wrote: From: Robert Richmond <rsrichm...@aol.com> Subject: [Histonet] formaldehyde neutralizers To: histonet@lists.utsouthwestern.edu Date: Wednesday, March 25, 2009, 10:06 AM Thanks, Tony Henwood! Your explanation of how to neutralize formaldehyde with ammonia is the only clear explanation of formaldehyde neutralization I've ever read. One more question: how does neutralization with sodium bisulfite work? What are the advantages and disadvantages of the two methods? Bob Richmond Samurai Pathologist Knoxville, Tennessee _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Wed, 25 Mar 2009 10:39:29 -0400 From: Alyssa Peterson <aly...@alliedsearchpartners.com> Subject: [Histonet] Histology Supervisor, Permanent Job, FREE MEDICAL BENEFITS! To: histonet@lists.utsouthwestern.edu Message-ID: <bbc6db3a0903250739i41b932b9mceec0e395e640...@mail.gmail.com> Content-Type: text/plain; charset=windows-1252 *Position of the Histology Supervisor* Do you want the benefits you deserve with the rewards you can see? Do you want the advancement, flexibility and resources to advance your career and provide the care that your patients need? **FREE medical and dental benefits, domestic partner benefits, excellent tuition reimbursement, continuing education and more!** *Description:* Supervise, coordinate and participate in providing laboratory services to meet the needs of patients as ordered by the medical staff and performed in accordance with defined standards and practices in general and unique to assigned section(s). *Day shift with variable start times - Monday - Friday* Bachelor of Science degree in Medical Technology or related biological science Six years of medical lab experience of which two years must be in particular section supervised. NYS licensure required HT, (ASCP) preferred *Montgomery**, **NY** area:* *60 miles from Danbury, CT* *60 miles from Newark, NJ* *60 miles from Scranton, PA* What a great opportunity! REMEMBER, THIS WON'T LAST. Interested? Please send resume in Microsoft Word format to: aly...@alliedsearchpartners.com **Please forward this email to anyone who you seems fit for this position, as the referral bonus for this position is $1000 if we place a person that you send to us in a position!** -- Alyssa Peterson Allied Search Partners O: 770.621.2639 ext. 4 F: 770.621.2640 ------------------------------ Message: 10 Date: Wed, 25 Mar 2009 07:42:44 -0700 From: "Martin, Gary" <gmar...@marshallmedical.org> Subject: [Histonet] Frozen section To: <histonet@lists.utsouthwestern.edu> Message-ID: <6ed9d4252f278841a0593d3d788af24c04dd5...@mailsvr.marshmed.local> Content-Type: text/plain; charset="us-ascii" We need to make a change in the way we presently account for our frozen section while doing them. Presently we receive the requisition with the first specimen, then pathology is responsible to account for any subsequent specimens. The problem is that the subsequent specimens are typically labeled poorly, and we are trying very hard to conform to the CAP guidelines. So ... when the specimens are not labeled in detail, it requires follow up calls to gain the proper information. I would like to know how other facilities are handling multiple frozen sections. Thanks Gary ------------------------------ Message: 11 Date: Wed, 25 Mar 2009 10:50:08 -0400 From: "McNabola, Angela" <lam...@bpthosp.org> Subject: RE: [Histonet] Frozen section To: "Martin, Gary" <gmar...@marshallmedical.org>, <histonet@lists.utsouthwestern.edu> Message-ID: <6fafe015e5ac6b4abaef2883a6f8a6d9016c6...@exch1.bpthosp.org> Content-Type: text/plain; charset="us-ascii" We require a form for each specimen. Yes, the subsequent forms may have less information (i.e. procedure being performed), but all have the patient id information, sample type and description, etc. They are treated as separate samples. -Angela Angela McNabola, MS,HT(ASCP)SLS, QIHC Manager Histology/Cytology Department of Pathology Bridgeport Hospital 267 Grant Street Bridgeport, CT 06610 phone: 203-384-4434 lam...@bpthosp.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martin, Gary Sent: Wednesday, March 25, 2009 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen section We need to make a change in the way we presently account for our frozen section while doing them. Presently we receive the requisition with the first specimen, then pathology is responsible to account for any subsequent specimens. The problem is that the subsequent specimens are typically labeled poorly, and we are trying very hard to conform to the CAP guidelines. So ... when the specimens are not labeled in detail, it requires follow up calls to gain the proper information. I would like to know how other facilities are handling multiple frozen sections. Thanks Gary _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Wed, 25 Mar 2009 10:55:13 -0400 From: Geoff McAuliffe <mcaul...@umdnj.edu> Subject: Re: [Histonet] Current books for Histotechnology Cc: "'Histonet@lists.utsouthwestern.edu'" <Histonet@lists.utsouthwestern.edu>, 'Ingles Claire ' <cing...@uwhealth.org> Message-ID: <49ca45d1.4060...@umdnj.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed A for atlases, I also like Wheater's FUNCTIONAL HISTOLOGY, or Gartner and Hiatt's COLOR ATLAS OF HISTOLOGY. I don't like Ross and Pawlina's HISTOLOGY, the third edition by Ross, Romrell and Kaye is much better and might be cheaper. Geoff Smith, Allen wrote: > For an atlas, Ross and Pawlina's HISTOLOGY, Wheater's FUNCTIONAL HISTOLOGY, > or Gartner and Hiatt's COLOR ATLAS OF HISTOLOGY are all good. They are also > more accurate than DiFiore. > -Allen a. Smith,Ph.D. > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire > Sent: Monday, March 23, 2009 11:55 AM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Current books for Histotechnology > > I would strongly recommend di Fiore's Atlas of Histology by Victor P. > Eroschenko. I don't know what edition its in now. I used it when I went > through my program. It is great for microscopic anatomy, especially when > combined with actual slide viewing. > Claire > > ________________________________ > > From: histonet-boun...@lists.utsouthwestern.edu on behalf of Jennifer > MacDonald > Sent: Fri 3/20/2009 3:45 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Current books for Histotechnology > > > > Our library has funds available to purchase books for the Histotechnology > program. The problem is that we need current books. We have the latest > Bancroft and Gamble. Any other suggestions for books that are newer than > 2000? I have suggested John Kiernan's latest. > By the way I did find a copy of Sheehan for $2,400!! > > Jennifer MacDonald > Education Coordinator, Histotechnician Training Program > Mt. San Antonio College > 1100 N. Grand Ave. > Walnut, CA 91789 > (909) 594-5611 ext. 4884 > jmacdon...@mtsac.edu > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ********************************************** ------------------------------ Message: 13 Date: Wed, 25 Mar 2009 11:40:34 -0400 From: "Weems, Joyce" <jwe...@sjha.org> Subject: RE: [Histonet] Frozen section To: "Martin, Gary" <gmar...@marshallmedical.org>, <histonet@lists.utsouthwestern.edu> Message-ID: <5d64396a0d4a5346bebc759022aaeaa53ea...@itsssxm01v6.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" We require a separate req for each. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martin, Gary Sent: Wednesday, March 25, 2009 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen section We need to make a change in the way we presently account for our frozen section while doing them. Presently we receive the requisition with the first specimen, then pathology is responsible to account for any subsequent specimens. The problem is that the subsequent specimens are typically labeled poorly, and we are trying very hard to conform to the CAP guidelines. So ... when the specimens are not labeled in detail, it requires follow up calls to gain the proper information. I would like to know how other facilities are handling multiple frozen sections. Thanks Gary _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ------------------------------ Message: 14 Date: Wed, 25 Mar 2009 11:46:03 -0400 From: "Barone, Carol " <cbar...@nemours.org> Subject: [Histonet] RNA and DNA yields from Laser Microdissection To: histonet@lists.utsouthwestern.edu Message-ID: <37e4bac017f57141af64faa5aeb04ce801471...@wlmmsx01.nemours.org> Content-Type: text/plain; charset=iso-8859-1 Histonet: I have a couple questions I would like to throw out to the experts: 1. Do you think higher yields of DNA /RNA in LMD are related to protocol, method differences from one instrument to another ( i.e. catapult verses,, gravity drop, verses, arcturus melt system...or more related to histologist v. research tech handling of the sample? 2. How do you feel about frozen verses paraffin as related to yield and quality in LMD/LCM? ------------------------------ Message: 15 Date: Wed, 25 Mar 2009 17:58:20 +0100 From: ne...@staff.uni-marburg.de Subject: Re: [Histonet] RNA and DNA yields from Laser Microdissection To: "Barone, Carol " <cbar...@nemours.org> Cc: histonet@lists.utsouthwestern.edu Message-ID: <20090325175820.h7xmiauajo48k...@home.staff.uni-marburg.de> Content-Type: text/plain; charset=ISO-8859-1; DelSp="Yes"; format="flowed" Hi Carol! My experience is, the yield depends on the protocol you use. I've done the LCM system from arcturus and the PALM-System from Zeiss. The highest yields had been for RNA and DNA by using the Pico Pure Kit (MSD) while doing LCM. This had been frozen sections. We isolated DNA from FFPE-Tissue from PALM and LCM by a conventional Proteinase K digest and phenol extraction. There is a paper of Fend and Specht at the Am. J. of Pathology, 2002 or 2003 describing these protocols. As long as the histopathologist or the tech are able to identify the "cell/tissue" of interest, it doesn't influence the yield/quality of the RNA/DNA. Frauke Zitat von "Barone, Carol " <cbar...@nemours.org>: > Histonet: I have a couple questions I would like to throw out to the experts: > > 1. Do you think higher yields of DNA /RNA in LMD are related to > protocol, method differences from one instrument to another ( i.e. > catapult verses,, gravity drop, verses, > arcturus melt system...or more related to histologist v. research > tech handling of the sample? > > 2. How do you feel about frozen verses paraffin as related to yield > and quality in LMD/LCM? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 64, Issue 44 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet