I use Mayer's Hematoxlyin for 30-45 secs. Wash for 2 minutes, blue in Ammonia Water, wash for 5 minutes, dehydrate clear and mount if it is DAB. If it is AEC I do the same except I rinse in distilled water before mounting with aqueous mount.
Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfranc...@uams.edu email -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Friday, March 27, 2009 9:23 AM To: ooi.ting.h...@nhc.com.sg; histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Different between Richard Allan and Harris & which one is better for IHC Hi all, I am adding my question to the below one. Which is better to use for IHC: Mayer's or just hematoxylin? Thanks in advance, Naira Message: 15 Date: Fri, 27 Mar 2009 09:50:42 +0800 From: ooi.ting.h...@nhc.com.sg Subject: [Histonet] Different between Richard Allan and Harris Hematoxylin Hi, I am interested to know what is the different between different brands of hematoxylin especially for Richard Allan and Harris. I am glad if there is any advice or suggestion on the choosing of hematoxylin. Welcome any suggestion of webpage that may tell the differences too. I am dealing with plastic resin section and doing a normal hematoxylin and eosin staining. Your advice is appreciated. Thank you very much! Regards, Ooi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
