We are experiencing difficulties with our H&E staining and other Special Stains. After the slides are stained the edges of the tissue look fragmented/chattered. Our pathologists also thought the sections looked like they might be too thick but we checked all of our microtomes for thickness settings and all are set at 4 micrometers. There seems to be several blotches of light staining throughout the tissue. This seems to be only on our small biopsies. We use McCormick Paraplast Extra for paraffin wax. Another bit information is that we recycle all of our xylene.
Is this a deparaffinization, processing, or a staining issue? Any help is appreciated. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgo...@clinlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet