Hi Boma:
Whether or not a saline or buffer flush was used before glut. fixation
is of little consequence to the effects of glut as a fixative.The
saline/buffer washout is just to remove blood. In my experience most
people overdo the washout.
I am curious as to what "disrupt the blood-brain barrier" means. The bbb
is a very complex entity with both physical and metabolic components.I
suspect the person who told you this is not well informed.
Geoff
Boma Fubara wrote:
Can someone explain to me the mechanism by which glutaraldehyde would
disrupt the blood brain barrier if the brain had not been flushed prior to
fixation?
Boma.
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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