Folks, I need some input on a problem we're seeing with some mouse brain samples. The samples are from new born mice P0 to P14 which have been fixed in 10% NBF (Fisher brand and well within expiration date) for 72 hours. They are then transferred to 70% etoh and processed on an 8 hour process. After the tissue is embedded, we are taking sagital sections, drying them overnight, and then baking overnight at 60C. They are then stained with H&E. This has been working great but now all of a sudden we have very light H&E staining, nuclear bubbling, and extensive tissue cracking. I also have noticed some formalin pigment.
My first thought is that there was difference in formalin or time in formalin but that is not the case. I also wondered if the tissue might be exposed to excessive heat. We checked all of the temperatures in our processor, embedding center, and water baths - all were within tolerance. Any ideas what might cause all three artifacts? Could it be from inadequate paraffin infiltration? Any help would be greatly appreciated! Thanks, Julie Julie Randolph-Habecker, Ph.D. Staff Scientist - Director Experimental Histopathology Shared Resource Fred Hutchinson Cancer Research Center 1100 Fairview Ave, N. DE-360 (Please note new location) Seattle WA 98109-1024 206-667-6119 jhabe...@fhcrc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet