It is obviously too late for this email to be useful in this case but I will offer a suggestion for future reference. When E histolytica in volves the liver it typically is in the form of an abscess. Look very carefulls at the specimen and make a wet prep smear of a small amount of tissue from anything that looks different than the normal brown appearing liver tissue. If you see anything soft or liquifactive yellowish or tan this would be ideal. Crush a speck of this tissue on a slide with a little saline and examine immediately with a lot of contrast. If E. histolitica are present you will see these cool little buggers, not much bigger than a histiocyte with small cookie cutter round nuckei and I tiny central dot crawling around the slide with purposeful motion and some containg RBCs. The encysted forms are a bit larger, rounder and contain 4 nucleoli. If recieved in saline it would be worth spinning down the saline an having a look at that. You can stain the slides as discribed above after looking at the wet mount.
Stephen Peters M.D. 201 847 0052 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 Phone and fax 201 847 7600 www.pathologyinnovations.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
