Nancy, Best advice I can offer is to ensure as much fixation as you can. We start with 30 minutes at 30oC and the ramp it up to 40oC for 30-60 minutes, rinse in 70% ethanol (5min)then continue microwave processing with isopropanol and wax. You may also consider decreasing the processing temperatures.
GI biopsies being quite small do not need over-the-top processing, but in my experience, fixation is still the key. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of magod...@aol.com Sent: Tuesday, 7 April 2009 10:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] gi microwave processing I am starting a gi lab and using a microwave processor for my first time. Any suggestions? SHUR Wave processor. Thanks, Nancy **************A Good Credit Score is 700 or Above. See yours in just 2 easy steps! (http://pr.atwola.com/promoclk/100126575x1221621488x1201450096/aol?redir =http:%2F%2Fwww.freecreditreport.com%2Fpm%2Fdefault.aspx%3Fsc%3D668072%2 6hmpgID %3D62%26bcd%3DAprilfooterNO62) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet