I use the Ultra View Detection kit on the Ventana Benchmark XT with the following protocol:
Deparaffinization CC1 Mild Mammaglobin - 32 min. Ultra Wash Hematoxylin - 4 min. Bluing - 4 min. We have very good results. Mammaglobin isn't expressed in every breast case either, so the control could be the culprit. We had to try a few tissues before we found one that yielded good results. Jodie Robertson, HT (ASCP) QIHC Pathology Sciences Medical Group Histology Day Supervisor 183 E. 8th Ave. Chico, CA 95926 530-891-6244 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Tuesday, April 07, 2009 10:36 AM To: 'Vickroy, Jim' Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] MAMMOGLOBIN I tried also this antibody on Ventana Benchmark. Pity, I had no results. Gudrun -----Ursprüngliche Nachricht----- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Vickroy, Jim Gesendet: Dienstag, 07. April 2009 17:54 An: 'Histonet@lists.utsouthwestern.edu' Betreff: [Histonet] MAMMOGLOBIN Has anyone used the DAKO predilute antibody, Mammoglobin, on the Ventana Benchmark XT? And if so.....could you share the protocol you used? Finally looking on the Dako website it appears that they have ready to use, could you share with me which antibody should be used with the Ventana I-view detection kit? Thanks Jim Vickroy BS, HT(ASCP) Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center 217-788-4046 vickroy....@mhsil.com This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet