I used to run a negative control per case or per block within any given case when doing IHC, but not for reagents. René J.
--- On Wed, 4/8/09, Sheila Haas <micropathl...@yahoo.com> wrote: From: Sheila Haas <micropathl...@yahoo.com> Subject: [Histonet] Negative IHC controls To: histonet@lists.utsouthwestern.edu Date: Wednesday, April 8, 2009, 2:57 PM I have a question concerning ANP.22570 on the CAP checklist. Could you all tell me how you are handling negative controls for IHC staining? The question actually states (and I've confirmed with CAP) that we should be running two types of negative controls. One for reagents and one for each antibody in a run. I'd like to know what the practice is. This seems very costly and time consuming. Thanks in advance! Sheila Haas Laboratory Supervisor Micro Path Laboratories _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet