Give me a shout in regards to cell pellets & TMA's.
There are several methods and some are less expensive then others.
Have a good one.
Lori



Beecher is usually difficult to deal with.histonet-requ...@lists.utsouthwestern.edu wrote:
Send Histonet mailing list submissions to
        histonet@lists.utsouthwestern.edu

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When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."


Today's Topics:

   1. Re: TMA for cell pellets? (Bernie Taupin)
   2. To all spam gourmets (V. Neubert)
   3. RE: I recommend NOT opening that Attachment: (Hugh Luk)
   4. RE: TMA for cell pellets? (Hartson, Louise)
   5. Re: Formula 83 users? (Rene J Buesa)
   6. Re: Negative IHC controls (Rene J Buesa)
   7. RE: Formula 83 users? (Blazek, Linda)
   8. Re: Spyware from an Attachment: (Geoff McAuliffe)
   9. Formula 83-Thank you! (Jacqueline Farnsworth)
  10. HISTOS 5 processor (FU,DONGTAO)
  11. Position Open In Mass (Alyssa Peterson)
  12. Re: HISTOS 5 processor (Robert Schoonhoven)
  13. Travelling Histotechs (Robert Schoonhoven)
  14. Regional Sales Manager position- West Coast
      (kris.caldw...@leica-microsystems.com)
  15. New lab, need some info (Putnam, Jodi)


----------------------------------------------------------------------

Message: 1
Date: Wed, 8 Apr 2009 22:50:26 -0700 (PDT)
From: Bernie Taupin <bernietau...@ymail.com>
Subject: Re: [Histonet] TMA for cell pellets?
To: Thom Jensen <tissuear...@hotmail.com>,
        louise_hart...@urmc.rochester.edu,      
histonet@lists.utsouthwestern.edu
Message-ID: <338565.20208...@web43513.mail.sp1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Recently, someone mentioned putting a library of protocols online, perhaps in 
the HistoNet archives. I'd like to second that emotion if its possible to make 
happen... What a great resource that would be!




________________________________
From: Thom Jensen <tissuear...@hotmail.com>
To: louise_hart...@urmc.rochester.edu; histonet@lists.utsouthwestern.edu
Sent: Thursday, April 9, 2009 1:27:18 AM
Subject: [Histonet] TMA for cell pellets?



Have you tried to embed your cell pellets into a tissue microarray?  Two 
companies I would recommend:
www.arraymold.com
www.beecherinstruments.com

Two of the best TMA products on the market.


cheers,

Date: Thu, 2 Apr 2009 12:05:39 -0400
From: louise_hart...@urmc.rochester.edu
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Embedding cell pellets


I am looking for a protocol for embedding cell pellets in paraffin.
Thanks,
Louise

Louise Hartson, BA
Senior Technical Associate
University of Rochester
louise_hart...@urmc.rochester.edu
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_________________________________________________________________
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------------------------------

Message: 2
Date: Thu, 09 Apr 2009 10:07:54 +0200
From: "V. Neubert" <histonet.nos...@vneubert.com>
Subject: [Histonet] To all spam gourmets
To: histonet@lists.utsouthwestern.edu
Message-ID: <49ddacda.8040...@vneubert.com>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

Please, stop spamming this list.
Comment if you know something useful. Otherwise keep it.
I don't mind what you had for breakfast in June 1st last year or your opinion on my clothes' colour.

Believe it or not, there IS a real life ( link: http://en.wikipedia.org/wiki/Real_life_(reality) ), and maybe you should check it out - it has way more to offer than waiting for responses to mock about.

Thanks.
Valentin

PS: What about a moderated board? Every inappropriate comment on HISTO TOPIC could be moved or even deleted, except in the spamming forum which can be found on nearly every board on the net.



------------------------------

Message: 3
Date: Wed, 8 Apr 2009 22:13:22 -1000
From: Hugh Luk <hlu...@msn.com>
Subject: RE: [Histonet] I recommend NOT opening that Attachment:
To: histonet <histonet@lists.utsouthwestern.edu>
Message-ID: <snt106-w30443621932d763b828123c3...@phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"




















Fellow histology and related fields professionals,











I'm not opening this.  From a call name of "u know 
(u_deserve_2_k...@yahoo.com)."  The last time I opened something like this, from a 
name like this, I ended up owing money for Viagra treatments for Panda bears or something 
like that.  It may be real, but it probably is spam.  There...has been...a lot...of 
it...recently!

By the way, there is no such thing as a "Virus-proof LINK!"  Not sure?  Just 
Google it.

If you need Spyware or Antivirus software, check your IT department. Also, CNET has free downloads of AdAware, or AVG antivirus 8.
And now for a joke (I think we all need it); A physician is doing his rounds and a nurse stops him in the 
hallway, "Doctor, can you sign these reports?"  "Fine" he says and proceeds to pull out a 
rectal thermometer from his coat pocket.  He looks at it in disbelief and exclaims, "Darn it!  Some a$$ 
has my pen!"

Respectfully,
Hugh Luk, HTL (ASCP)
Cancer Research center of Hawaii
Pathology shared resources lab manager
KP- Hawaii


Date: Wed, 8 Apr 2009 21:41:35 -0700
From: u_deserve_2_k...@yahoo.com
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Spyware from an Attachment:

Dear Listers,

If you ever opened any strange attachments from this list, you should really 
consider scanning your Hard Disk for spyware. There is a great free anti-spyware 
program we use, which you can get at http://DELETED<----that is a virus-proof 
link, by the way.



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_________________________________________________________________
Quick access to your favorite MSN content and Windows Live with Internet Explorer 8. http://ie8.msn.com/microsoft/internet-explorer-8/en-us/ie8.aspx?ocid=B037MSN55C0701A

------------------------------

Message: 4
Date: Thu, 9 Apr 2009 08:29:23 -0400
From: "Hartson, Louise" <louise_hart...@urmc.rochester.edu>
Subject: RE: [Histonet] TMA for cell pellets?
To: "Bernie Taupin" <bernietau...@ymail.com>,   "Thom Jensen"
        <tissuear...@hotmail.com>,        <histonet@lists.utsouthwestern.edu>
Message-ID:
        <1089a756b010074ca09e65cd92401608018a8...@medmail.urmc-sh.rochester.edu>
        
Content-Type: text/plain;       charset="iso-8859-1"

Thank you to everyone who responded!!  I was given the cell pellets already in 
the matrix so I only had to process!!  It was easier than the mouse tissues!!  
Since we do so few 20-40 at a time I do everything by hand...no automation in 
our lab!!


-----Original Message-----
From: Bernie Taupin [mailto:bernietau...@ymail.com]
Sent: Thu 4/9/2009 1:50 AM
To: Thom Jensen; Hartson, Louise; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] TMA for cell pellets?
Recently, someone mentioned putting a library of protocols online, perhaps in the HistoNet archives. I'd like to second that emotion if its possible to make happen... What a great resource that would be!




________________________________
From: Thom Jensen <tissuear...@hotmail.com>
To: louise_hart...@urmc.rochester.edu; histonet@lists.utsouthwestern.edu
Sent: Thursday, April 9, 2009 1:27:18 AM
Subject: [Histonet] TMA for cell pellets?



Have you tried to embed your cell pellets into a tissue microarray?  Two 
companies I would recommend:
www.arraymold.com
www.beecherinstruments.com

Two of the best TMA products on the market.


cheers,

Date: Thu, 2 Apr 2009 12:05:39 -0400
From: louise_hart...@urmc.rochester.edu
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Embedding cell pellets


I am looking for a protocol for embedding cell pellets in paraffin.
Thanks,
Louise

Louise Hartson, BA
Senior Technical Associate
University of Rochester
louise_hart...@urmc.rochester.edu
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_________________________________________________________________
Hotmail® is up to 70% faster. Now good news travels really fast.
http://windowslive.com/online/hotmail?ocid=TXT_TAGLM_WL_HM_70faster_032009_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





------------------------------

Message: 5
Date: Thu, 9 Apr 2009 06:56:21 -0700 (PDT)
From: Rene J Buesa <rjbu...@yahoo.com>
Subject: Re: [Histonet] Formula 83 users?
To: Histonet <Histonet@lists.utsouthwestern.edu>, Jacqueline
        Farnsworth <jacqueline.farnswo...@cls.ab.ca>
Message-ID: <613905.80912...@web65715.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Jacqueline:
Formula 83 is a naphthenic hydrocarbon that cannot be used with coverslippers, 
is recyclable and while some people have been using it for more than 20 years, 
others find it irritant.
René J.

--- On Wed, 4/8/09, Jacqueline Farnsworth <jacqueline.farnswo...@cls.ab.ca> 
wrote:

From: Jacqueline Farnsworth <jacqueline.farnswo...@cls.ab.ca>
Subject: [Histonet] Formula 83 users?
To: "Histonet" <Histonet@lists.utsouthwestern.edu>
Date: Wednesday, April 8, 2009, 12:31 PM

HI all.
I have searched the Histonet archives and found a lot of very positive reviews
regarding Formula 83. I am wondering if anyone has encountered any issues with
using Formula 83 on a stainer and then xylene on a tape coverslipper.  Are there
any miscibility issues? Are you soaking in xylene prior to tape coverslipping
required? Any helpful hints/tips/tricks?

Thank you in advance.


Jacqueline Farnsworth
Anatomic Pathology, Tech III
Foothills Medical Centre
Calgary Laboratory Services

Ph: 403-944-1578
Fax: 403-944-4748
P Please consider the environment before printing this email.

________________________________
This message and any attached documents are only for the use of the intended
recipient(s), are confidential and may contain privileged information. Any
unauthorized review, use, retransmission, or other disclosure is strictly
prohibited. If you have received this message in error, please notify the sender
immediately, and then delete the original message. Thank you.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 6
Date: Thu, 9 Apr 2009 06:58:45 -0700 (PDT)
From: Rene J Buesa <rjbu...@yahoo.com>
Subject: Re: [Histonet] Negative IHC controls
To: histonet@lists.utsouthwestern.edu, Sheila Haas
        <micropathl...@yahoo.com>
Message-ID: <292901.13223...@web65712.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

I used to run a negative control per case or per block within any given case 
when doing IHC, but not for reagents.
René J.

--- On Wed, 4/8/09, Sheila Haas <micropathl...@yahoo.com> wrote:

From: Sheila Haas <micropathl...@yahoo.com>
Subject: [Histonet] Negative IHC controls
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, April 8, 2009, 2:57 PM

I have a question concerning ANP.22570 on the CAP checklist. Could you all
tell me how you are handling
negative controls for IHC staining? The question actually states (and I've
confirmed with CAP) that we should be running two types of negative controls.
One for reagents and one for each antibody in a run. I'd like to know
what the practice is. This seems very costly and time consuming. Thanks in
advance!
Sheila Haas
Laboratory Supervisor
Micro Path Laboratories



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Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 7
Date: Thu, 9 Apr 2009 10:14:48 -0400
From: "Blazek, Linda" <lbla...@digestivespecialists.com>
Subject: RE: [Histonet] Formula 83 users?
To: "'rjbu...@yahoo.com'" <rjbu...@yahoo.com>, Histonet
        <Histonet@lists.utsouthwestern.edu>, Jacqueline Farnsworth
        <jacqueline.farnswo...@cls.ab.ca>
Message-ID:
        
<5a2bd13465e061429d6455c8d6b40e3908778d1...@ibmb7exchange.digestivespecialists.com>
        
Content-Type: text/plain; charset="iso-8859-1"


With all due respect René; I have been using Formula 83 with my coverslipper 
with no problems at all though have a glass coverslipper not a tape.
Linda

Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 293-4424 ext 7118
Email: lbla...@digestivespecialists.com

-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Thursday, April 09, 2009 9:56 AM
To: Histonet; Jacqueline Farnsworth
Subject: Re: [Histonet] Formula 83 users?

Jacqueline:
Formula 83 is a naphthenic hydrocarbon that cannot be used with coverslippers, 
is recyclable and while some people have been using it for more than 20 years, 
others find it irritant.
René J.

--- On Wed, 4/8/09, Jacqueline Farnsworth <jacqueline.farnswo...@cls.ab.ca> 
wrote:

From: Jacqueline Farnsworth <jacqueline.farnswo...@cls.ab.ca>
Subject: [Histonet] Formula 83 users?
To: "Histonet" <Histonet@lists.utsouthwestern.edu>
Date: Wednesday, April 8, 2009, 12:31 PM

HI all.
I have searched the Histonet archives and found a lot of very positive reviews
regarding Formula 83. I am wondering if anyone has encountered any issues with
using Formula 83 on a stainer and then xylene on a tape coverslipper.  Are there
any miscibility issues? Are you soaking in xylene prior to tape coverslipping
required? Any helpful hints/tips/tricks?

Thank you in advance.


Jacqueline Farnsworth
Anatomic Pathology, Tech III
Foothills Medical Centre
Calgary Laboratory Services

Ph: 403-944-1578
Fax: 403-944-4748
P Please consider the environment before printing this email.

________________________________
This message and any attached documents are only for the use of the intended
recipient(s), are confidential and may contain privileged information. Any
unauthorized review, use, retransmission, or other disclosure is strictly
prohibited. If you have received this message in error, please notify the sender
immediately, and then delete the original message. Thank you.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 8
Date: Thu, 09 Apr 2009 10:11:11 -0400
From: Geoff McAuliffe <mcaul...@umdnj.edu>
Subject: Re: [Histonet] Spyware from an Attachment:
To: u know <u_deserve_2_k...@yahoo.com>
Cc: histonet@lists.utsouthwestern.edu
Message-ID: <49de01ff....@umdnj.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

Dear List:

I strongly suggest that you NOT use any links offered by people with no name and no affiliation. Yes, there are good spyware detection programs available at no cost (Adaware for example) but I don't think this is one of them. There are at least 3 potential saboteurs on this list.

Geoff

u know wrote:
Dear Listers,

If you ever opened any strange attachments from this list, you should really 
consider scanning your Hard Disk for spyware. There is a great free anti-spyware 
program we use, which you can get at http://tinyurl.com/ynupj4 <----that is a 
virus-proof link, by the way.



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Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet







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