Peggy, If your microtome will hold a sharp D profile or a tungsten carbide blade, you might be able to get consistent sections. I find that EDTA decalcification hardens the tissue ( as if it wasn't hard enough already). Also, extending the times for the paraffin infiltration is helpful and soaking the block in icy/soapy detergent in the freezer works on some samples.
~ VIcki _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
