Hi, I want to ask this questions because I read different recipes in preparaing 
fixative solution...

Here we perfuse animals with 0.9% saline, then 4% PFA in 0.1M PB...that is not 
a physiological solution...
I see other people using 4% PFA in PBS.... will this lead to different brain 
shrinkage?

Thanks.

2009-05-05 



TF 
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