As you point out TEM would be one way. Perhaps Frozen sections could allow you to visualize iodine crystals, but completely rule out the paraffin way because the iodine will dissolve during processing. René J.
--- On Tue, 5/5/09, Connolly, Brett M <brett_conno...@merck.com> wrote: From: Connolly, Brett M <brett_conno...@merck.com> Subject: [Histonet] Stain for iodine? To: histonet@lists.utsouthwestern.edu Date: Tuesday, May 5, 2009, 10:19 AM Histonetters, We have tissue that contains a iodinated nanosphere CT contrast agent and would like to identify the cells that took up said agent. Other than doing TEM does anyone have a suggestion for a stain we could use to visualize the iodine? Can be for either frozen or paraffin sections. Thanks, Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_conno...@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet