Hello:
Does anyone have any experience with using the Snap-Frost rapid freezing
system from Alphelys (France)? Our application is predominantly for
tissue banking though we do a booming business in intraoperative
diagnosis as well. The company and one review article {Virchow Arch
(2008) 452:305-312} claim good RNA/DNA recovery from OCT embedded,
isopentane frozen tissue using the system. They also claim good to
excellent morphology at -80 in isopentane compared to liquid nitrogen at
-190 and especially compared to CO2 freezing. Can anyone confirm or
refute the claims? Does anyone know of a US based supplier of the
Snap-Frost system? Thanks.
Joe Galbraith
University of Iowa Tissue Repository
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