Agree; I guess the difference is that FNAC and Micro samples haven't been subjected to processing like the tissue block sections. The tissue stains have been shown to work on something that has been fixed, dehydrated, set in hot wax, cut, rehydrated and then stained. The FNAC and Micro samples may or may not have been fixed (although I concede air drying is a form of fixation) and the stains used on them have been shown to work.
The logic that these techniques are interchangeable is not only flawed but (oxy)moronic. Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 06 May 2009 17:24 To: histonet@lists.utsouthwestern.edu; Angela Bitting Subject: Re: [Histonet] Gram & AFB staining FNA smears The "doc" is wrong, otherwise your histology sections to be stained with Gram & AFB should also be sent to micro. Perhaps you will not have to do them anymore. Your "doc's" reasoning is purely oxymoronic. René J. --- On Wed, 5/6/09, Angela Bitting <akbitt...@geisinger.edu> wrote: From: Angela Bitting <akbitt...@geisinger.edu> Subject: [Histonet] Gram & AFB staining FNA smears To: histonet@lists.utsouthwestern.edu Date: Wednesday, May 6, 2009, 9:22 AM The subject of staining FNA smears with AFB and Gram stains in Histology vs in Micro came up today. Is there a reason that FNAs can't be stained the same way we stain our tissue sections? One of our docs was under the impression that it's not acceptable to use the staining methods we use in our Histology lab. I don't know what method Micro labs use, so I was hoping someone could shed some light on this subject for me. Thanks, as always, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet