Gudrun,Ki67 as stained by SP6 rabbit monoclonal antibody survives quite long formaldehyde fixation. Up to 2 months of fixation, the staining intensity is as strong as after 1 day of formaldehyde fixation. Even after one year in formalin there is still staining of this marker. I don't know what the status of Ki67 will be after too short fixation. Cheers,ChrisChris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The NetherlandsDate: Sat, 9 May 2009 20:34:39 +0200 From: "Gudrun Lang" <[email protected]> Subject: [Histonet] KI67 test for fixation? To: [email protected] Hi all!
Can anybody explain the correlation of KI-67 stainability and the status of formaldehyde-fixation? I cannot remember, if an under- or overfixed tissue shows the positive KI67 nuclei. And I don't remember, if this test is still valuable. Please help, Gudrun _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
