Clone Mec13.3 (BD Pharmingen sells it among others) works great on PFA fixed frozen tissues but you must use trypsin as an antigen retrieval step. I do 30 min at 37 deg C. Depending on your tissue that may need some adjusting.
------Original Message------ From: TF Sender: [email protected] To: histonet ReplyTo: [email protected] Sent: May 11, 2009 7:41 AM Subject: [Histonet] Anti -rat CD31 Dear All Just wonder whether you know any anti-rat CD31 that work on PFA-fixed tissues - frozen section ? It seems all of them just work on acetone fixed tissues! The tissue quality can not be promised ! Even you try antigen retrieval, it wont work? 2009-05-11 TF _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
