Yes, we have had problems with our IP stains (primarily C4d) when performed on rushed transplant biopsies. The paraffin-embedded tissue does not hold up well to enzyme digestion when it's not properly fixed. After educating our transplant surgeons they are perfectly happy to wait until the next day. In situations where an immediate read is needed, two cores can be taken; one for rush morphology and one for overnight fixation and subsequent immunostaining.
Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimens Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Gudrun Lang" <[email protected]> 5/11/2009 10:52 AM >>> Hi all! Until now we were lucky to have no short-processing-protocols to deal with. Now we start with transplantation kidney biopsies and a two-hour-protocol on a VIP. Receiving biopsy – 30 min NBF – then VIP: 30 min NBF 40°C, 15 min 70% alk, 15 min 80% alk, 15 min 96% alk, 15 min 100% alk, 30 min paraffin We do HE and specialstains ( Jones, PAS, EvG, Congored, Trichrom SFOG) and immunofluorescenc (Ig) and immunohistochemistry (C4d, HLA, Polyomavirus). If anyone have bad experiences with the short protocols, would you mind to share those with me? Regarding cutting and staining. What are the points, I have to take care of? Good experiences are also welcome. Bye Gudrun _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
