I do not routinely perform decalcified bone preparations as I predominantly utilize undemineralized resin (MMA) applications, so maybe someone else can better respond to this question. However, I would say that you could store your bone in 70% EtOH until you are ready to process, but the length of time in solution storage may cause you problems down the road since you have already decalcified. I think that a prolonged storage time might make your bone hard again....if that makes any sense....and cause you a fit later when you go to the microtome. Typically, I store undemineralized bones at either 10% NBF or 70% EtOH depending upon what I care to see at the microscope.
Jack > Date: Thu, 21 May 2009 10:46:59 -0300 > From: redw...@ucb.br > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Bone! > > Friends, > > Can someone recommend to me how to store bones (juvenile rat femur) that I > have fixed and decalcified, but am not yet ready to cut (they're for > vibratome, so not embedded). > > Thanks! > > Redward. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
