That's a 'harsh' fixative; used it years and years ago as it mordants the haematoxylin. I think that I still stand by the harsh effects of that fixative on coagulating protein and that the culprit is precisely that; coagulated protein from using a additive fixative (as you know formalin is a coagulant, non-additive fixative, normally).
Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Christine I. Braaten Sent: 22 May 2009 17:36 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cloudy formalin in vip tissue processor Thanks to all who have replied to my query. I probably should have mentioned that we use zinc formalin in our processor and as our primary fixative. I have spoken to both Sakura and Anatech (supplier of zinc formalin) and feel more confused. Anatech says it's a change in pH that causes the zinc to precipitate and that xylene will cause a whole lot more problems than a little cloudiness. Sakura thinks the zinc formalin is the problem. We clean the retort with the cleaning program every morning and make sure to dry the retort so I'm not sure how xylene would get into the formalin. If this brings up any other issues I would appreciate more feedback. Thanks CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet