Hello, Sorry to be a pest, but normally responses come quite quick to queries so I'm figuring that maybe my last query never registered (this being the wake of Memorial Day weekend and all...).
Has anyone out there tried DAB on sections and thereafter stained with luxol fast blue-cresyl violet? I'm looking at neurogensis in the brain of fish and want to be able to get a good idea of which layers the DAB+ nuclei are lying in. Any other suggestions for a counterstain that can be used on DAB stained sections? I'm adjusting the DAB color with Cobalt Chloride, which should change it from brown to blue-black---maybe not the best idea with LFB. What about when using copper or nickle? Thanks -- Tyrone Genade http://tgenade.freeshell.org email: tgen...@freeshell.org tel: +27-84-632-1925 (c) ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
