There's a silverstain for melanin and other argentaffine granules: Fontana-Masson. The principle is based on the reducing effect of melanin on silverdiaminions. You can find the procedure in the AFIP manual. Gudrun
-----Ursprüngliche Nachricht----- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Medea McGraw Gesendet: Mittwoch, 27. Mai 2009 20:36 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Melanin / melanocytes staining method tintorial or IHC Craig, I was searching the archives on the histonet concerning the Dopa Oxidase stain. I have not performed this stain but have done the Schmorls stain. I was wondering if you had a particular method/protocol that you use for this stain? Are there any kits available or is it cheaper to do buying the reagents separately? Any suggestions would be greatly appreciated. Regards, Medea -- Medea J. McGraw BS, HT, HTL(ASCP) CM IHC Research Histotechnologist Phenopath Laboratories Phone: 206-374-9000 x1033 Fax: 206-374-9009 This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
