In the lab I am currently working the microtome is often used to cut speciments of hard shell snails and other mterials that most of the times have calcifications that are difficult to retrieve, so most times tissues have to go to further decalcification and I believe that this could have caused an unvisable deviation on the microtome since, we have striations in all parafin sections, even with new blades and soft tissues, that compromise a good histology section for evaluation of tissues. Anyone has any comments to do on a possible deviation a microtome can get. Any suggestions or test to prove this possibility? If you were suspecting from such a problem in your microtome, what would you do? contact the manufecturer? Anyone had this problem? Or is a deviation of the microtome possible when it is used often with calcified tissues?
-- Ana Resendes DVM, MSc, PhD, Veterinary Pathologist Postdoctoral DRCT-fellow research scientist Centro de Investigação de Recursos Naturais (CIRN) Laboratório de Microscopia e Histologia Departamento de Biologia, Universidade dos Açores. Rua da Mãe de Deus, 58 - Apartado 1422 P - 9501-801 Ponta Delgada (Açores) Portugal Tel. (+351) 296 650 111 Fax (+351) 296 650 100 http://www.uac.pt/~pherg/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet