Our Sakura coverslipper is from 1994 (yep...I win the prize!!). When we have had those "brown" spots, we have discovered that they are actually dry areas. I would look at the last little white roller bar on the track. The springs(2) that keeps it in place, and keeps that roller bar nice and flat to squish out the air bubbles, may be going bad. When we have replaced the springs, our brown spots have been eliminated. In addition, we have to have the volume of xylene at about 6 drops to accomadate the cytology smears, which does leave the slides a little on the wet side. So we just put them under the hood for a couple minutes to dry.
Hope this helped! Happy Friday!!! Lynette >>> Jackie M O'Connor <Jackie.O'con...@abbott.com> 05/29/09 9:52 AM >>> I have an older Sakura coverslipper, circa 1997ish. We have noticed an increasing number of brown spots on tissue sections recently. This does not occur if the same slides are covered with glass coverslips. It does not look like a drying artefact, occurs randomly throughout tissue types, location on tissue, i.e., edge, center. I've even showed these spots to the rep who admitted she has never seen this artefact before. We use only the Tissue Tek tape, we maintain the equipment on a regular basis. The xylene flow is good. Our only problem are these random brown spots. Any suggestions? Jackie O' "Karin Groeger" <kgroe...@uslabs.net> Sent by: histonet-boun...@lists.utsouthwestern.edu 05/29/2009 08:19 AM To "Scott" <lsc...@sfcn.org>, <Histonet@lists.utsouthwestern.edu> cc Subject RE: [Histonet] Sakura film coverslipper xylene residue issue Hi Scott, We place ours in front of a fan to dry if we are in a hurry, even if we air dry them we do not have a problem with the xylene residue, perhaps you have a bad batch, check your lot # and call Sakura. We coverslip over 1,000 slides a day and have not seen this. Karin Groeger Histology Supervisor US LABS, Irvine,CA 949-450-0145 ext. 649 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott Sent: Thursday, May 28, 2009 10:03 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura film coverslipper xylene residue issue Hi, We have a newer Sakura film coverslipper, does anyone have experience with this piece of equipment. I have a problem with the slides having a light xylene residue, after the slides come off of the coverslipper. How do you dry them? If I leave the slides on the coverslipper to air dry the xylene residue is noticeable. If I lightly wipe off all of the slides as they come off the coverslipper they look clean. How do you guys do it? I think it is a waste of time to wipe off all of the slides. I don't think the xylene flow is too high. Thanks for your help, Scott Hendricksen HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This message, including any attachments, may contain CONFIDENTIAL AND/OR LEGALLY PRIVILEGED information. The information is intended for use by the individual named above and may not be disseminated to any other party without US LABS' written permission. If you are not the intended recipient, or the employee or agent responsible for delivering this information to the intended recipient, you are hereby notified that any dissemination, disclosure, distribution, copying or taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this information in error, please notify US LABS immediately at 1-888-450-0145 attn: Compliance Department to arrange for return of this message including all attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
