You wrote: Hi all - I'm looking for a good antibody to use on mouse tissue (frozen)
to look for CD11c - does anyone have a suggestion for a good antibody source? Thanks in advance - Melissa We us CD11c, HL3 clone, Armenian Hamster anti Mouse. Negative control is Armenian Hamster IgG1 from BD Pharmingen/Invitrogen. Tissues are fresh frozen sections fixed with our favorite acetone/absolute ethanol fixative, although you can use 4C acetone for 10 min on overnight air dried sections. I will be happy to send the other fixative recipe if you want it. Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet