Robert If you are using formalin fixed paraffin embedded human material, Sigma have a very nice mouse monoclonal that works beautifully. The cat no. is M-4403 (clone HM-2).
Cheers Jim Jim Manavis Laboratory Manager Hanson Institute Centre for Neurological Diseases IMVS, Adelaide, SA, 5000 Australia Phone: 61-08-8222-3668 / 0401120697 FAX: 61-08-8222 3392 email: jim.mana...@imvs.sa.gov.au Disclaimer: Not this little black duck! -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lott, Robert Sent: Saturday, 30 May 2009 2:05 AM To: histonet@lists.utsouthwestern.edu Cc: Christa Hladik Subject: [Histonet] MAP-2 antibody Hi Everyone, One of our pathologists is interested in MAP-2 IHC for the following application: Microtubule Associated Protein-2 Is a Sensitive Marker of Primary and Metastatic Neuroblastoma. Krishnan C, Heerema-McKenney A, Arber DA. Department of Pathology, Stanford University, Stanford, CA. Comment: Microtubule associated protein-2 (MAP-2) is a protein expressed in high levels in cells derived from the neural crest. It is a very robust marker of neuronal differentiation and has been used most often in evaluating neuronal differentiation in tumors of the central nervous system. MAP-2 showed comparable sensitivity in staining primary neuroblastomas (pre- and post-treatment samples) as compared to synaptophysin, chromogranin, CD56, and beta-catenin. In contrast to other markers of neuroblastoma, MAP-2 did not show significant cross reactivity to native bone marrow precursors, thus eliminating a potential source of confusion. Does anyone use this antibody for this or like applications.... I have looked and am "fairly" sure that there is not an IVD available, but would still be interested in knowing which antibody you use. Thanks, Robert L. Lott, HTL(ASCP) / Manager, Anatomic Pathology Trinity Medical Center/ LabFirst/ 800 Montclair Road / Birmingham, AL 35213/ 205-592-5387 -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet